Department of Chemistry and Institutes of Biomedical Sciences, Fudan University, Shanghai, 200433, PR China.
Analyst. 2020 Jun 7;145(11):3814-3821. doi: 10.1039/c9an02572c. Epub 2020 Mar 27.
Diarrheagenic Escherichia coli as an enteropathogen has caused serious public safety problems, especially in children. A fast, easy-to-use, high-throughput method is urgently needed for diarrheagenic Escherichia coli strain identification and monitoring. In this study, we developed a portable microfluidic device based on circular fluorescent probe-mediated isothermal nucleic acid amplification for accurate and rapid detection of twelve virulence factor genes of five diarrheagenic Escherichia coli strains for point-of-care testing. This microfluidic system showed excellent performance in identifying five diarrheagenic Escherichia coli strains within 60 min, when applied in 67 clinical samples collected from hospitals for identifying diarrheagenic Escherichia coli, with a good clinical sensitivity (96.9%), specificity (97.1%) and stability (CV < 5.0%). This integrated microfluidic system is a promising diagnostic tool for applications in the identification and monitoring of diarrheagenic Escherichia coli epidemics worldwide, particularly in developing countries.
产毒性大肠杆菌作为一种肠道病原体，已经造成了严重的公共安全问题，尤其是在儿童中。因此，非常需要一种快速、易用、高通量的方法来鉴定和监测产毒性大肠杆菌菌株。在这项研究中，我们开发了一种基于圆形荧光探针介导的等温核酸扩增的便携式微流控装置，用于即时检测五种产毒性大肠杆菌菌株的 12 种毒力因子基因。该微流控系统在 60 分钟内对五种产毒性大肠杆菌菌株的鉴定表现出了优异的性能，当应用于从医院采集的 67 份临床样本中鉴定产毒性大肠杆菌时，具有良好的临床灵敏度（96.9%）、特异性（97.1%）和稳定性（CV<5.0%）。这种集成的微流控系统是一种很有前途的诊断工具，可用于鉴定和监测世界各地的产毒性大肠杆菌流行情况，特别是在发展中国家。
