Department of Chemistry and Institutes of Biomedical Sciences , Fudan University , 2005 Songhu Road , Shanghai 200433 , P. R. China.
National Research Center for Exotic Animal Diseases , China Animal Health and Epidemiology Center , 369 Nanjing Road , Qingdao , Shandong 266032 , P. R. China.
ACS Sens. 2019 Nov 22;4(11):3066-3071. doi: 10.1021/acssensors.9b01731. Epub 2019 Oct 24.
The outbreak of African swine fever has brought serious public safety problems to the world, and there is a need for reliable diagnostic tools to prevent and control the epidemic. Here, we successfully established a portable microfluidic-circular fluorescent probe-mediated isothermal nucleic acid amplification (CFPA) system for the rapid, high-throughput, accurate, and sensitive detection of African swine fever virus at point-of-care settings. This detection system has a detection limit of 10 copies/μL, good stability (C.V. < 5%), and 92.73% sensitivity, and 100% specificity when tested on 220 pig samples collected for the diagnosis of African swine fever virus, with a median time to threshold of 10.8 min. This novel integrated diagnostic tool is urgently needed and has promising applications for the monitoring and control of African swine fever epidemics worldwide and, in particular, the serious outbreak in China.
非洲猪瘟的爆发给世界带来了严重的公共安全问题，因此需要可靠的诊断工具来预防和控制疫情。在这里，我们成功地建立了一种便携式微流控-圆形荧光探针介导的等温核酸扩增（CFPA）系统，可在即时检测环境中快速、高通量、准确和灵敏地检测非洲猪瘟病毒。该检测系统的检测限为 10 拷贝/μL，具有良好的稳定性（<5%CV），在对 220 份用于诊断非洲猪瘟病毒的猪样本进行测试时，灵敏度为 92.73%，特异性为 100%，中位时间为 10.8 分钟。这种新型集成诊断工具是迫切需要的，对于监测和控制全球范围内的非洲猪瘟疫情具有广阔的应用前景，特别是在中国爆发的严重疫情。
