Li Dongfeng, Zhang Kun, Pan Zaixu, Yu Minli, Lu Yinglin, Wang Guiying, Wu Junfeng, Zhang Jin, Zhang Kangning, Du Wenxing
College of Animal Science & Technology, Nanjing Agricultural University, Nanjing, China.
Animal Husbandry Research Institute, Beijing Sanyuan Breeding Technology Co, Ltd, China.
Anim Sci J. 2020 Jan-Dec;91(1):e13326. doi: 10.1111/asj.13326.
Antibiotics stimulate the growth of animals but result in drug residues and bacterial resistance. In this study, the negative effect of antibiotics on abdominal fat deposition was evaluated in broilers. The results showed that adding both chlortetracycline (50 g/1,000 kg) and tylosin (50 g/1,000 kg) significantly increased abdominal fat weight, abdominal fat percentage (p < .05), and triglyceride and cholesterol levels (p < .05) in blood. Also, both products synchronously stimulated intestinal absorption and synthesis of liver fat. The expression levels of the peroxisome proliferator-activated receptor γ (PPARγ), diacylgycerol acyltransferase 2 (DGAT2), lipoprotein lipase (LPL), and fatty acid-binding protein (FABP4) genes in abdominal fat tissue significantly increased (p < .05 or 0.01) when antibiotics were added to the feed. However, no significant difference was found in expression of the fatty acid synthesis (FAS) or acetyl CoA carboxylase (ACC) genes. Further in vitro study results revealed that antibiotics had no effect on fat content or the related gene expression levels in preadipocytes. In summary, the antibiotics induced fat deposition in adipose tissues by activating extracellular absorption of fatty acids from intestinal absorption and synthesis of liver fat. However, it shows no direct regulation by adipose tissue.
抗生素可促进动物生长，但会导致药物残留和细菌耐药性。在本研究中，评估了抗生素对肉鸡腹部脂肪沉积的负面影响。结果表明，添加金霉素（50克/1000千克）和泰乐菌素（50克/1000千克）均显著增加了腹部脂肪重量、腹部脂肪百分比（p<0.05）以及血液中的甘油三酯和胆固醇水平（p<0.05）。此外，这两种产品均同步刺激了肠道对脂肪的吸收以及肝脏脂肪的合成。当在饲料中添加抗生素时，腹部脂肪组织中过氧化物酶体增殖物激活受体γ（PPARγ）、二酰基甘油酰基转移酶2（DGAT2）、脂蛋白脂肪酶（LPL）和脂肪酸结合蛋白（FABP4）基因的表达水平显著升高（p<0.05或0.01）。然而，脂肪酸合成（FAS）或乙酰辅酶A羧化酶（ACC）基因的表达未发现显著差异。进一步的体外研究结果表明，抗生素对前脂肪细胞中的脂肪含量或相关基因表达水平没有影响。总之，抗生素通过激活肠道对脂肪酸的吸收以及肝脏脂肪的合成所导致的细胞外脂肪酸吸收，诱导了脂肪组织中的脂肪沉积。然而，它并未显示出对脂肪组织的直接调控作用。
