Li Yantao, Wang Boyu, Wang Zhaochuan, Wen Jintian, Zhou Tianle, Tang Jiahao, Li Zhenhui
State Key Laboratory of Swine and Poultry Breeding Industry, Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou 510642, China.
Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture and Rural Affair, South China Agricultural University, Guangzhou 510642, China.
Animals (Basel). 2025 Mar 26;15(7):951. doi: 10.3390/ani15070951.
The G0/G1 switch gene 2 () has been shown to be involved in cell proliferation, apoptosis, and differentiation in mammals. However, its function in poultry is not fully understood, especially in preadipocytes of chickens. This study aimed to establish a knockout preadipocyte cell line in chickens through CRISPR/Cas9 technology and to thoroughly investigate the impact of on chicken preadipocyte proliferation, apoptosis, and differentiation. To explore the involvement of in chicken preadipocyte growth and development, transcriptome sequencing was performed. The results demonstrated that was successfully deleted using the CRISPR/Cas9 system. knockout significantly inhibited the differentiation of chicken preadipocytes while promoting their proliferation. Additionally, although knockout exhibited a pro-apoptotic effect, it was relatively mild, primarily reflected in an increased proportion of early apoptotic cells. deletion significantly affected the expression of important genes related to lipid metabolism, cell cycle control, and signaling pathways, based on transcriptomic analysis. In conclusion, our findings suggest that performs a critical role in regulating chicken preadipocyte differentiation, proliferation, and apoptosis. This research offers valuable new insights into the molecular mechanisms and control of in the growth and development of chicken preadipocytes.
G0/G1转换基因2（）已被证明参与哺乳动物的细胞增殖、凋亡和分化。然而，其在家禽中的功能尚未完全了解，尤其是在鸡的前脂肪细胞中。本研究旨在通过CRISPR/Cas9技术建立鸡的敲除前脂肪细胞系，并深入研究对鸡前脂肪细胞增殖、凋亡和分化的影响。为了探究在鸡前脂肪细胞生长发育中的作用，进行了转录组测序。结果表明，利用CRISPR/Cas9系统成功删除了。敲除显著抑制了鸡前脂肪细胞的分化，同时促进了它们的增殖。此外，尽管敲除表现出促凋亡作用，但相对较轻，主要表现为早期凋亡细胞比例增加。基于转录组分析，缺失显著影响了与脂质代谢、细胞周期调控和信号通路相关的重要基因的表达。总之，我们的研究结果表明在调节鸡前脂肪细胞分化、增殖和凋亡中发挥着关键作用。本研究为在鸡前脂肪细胞生长发育中的分子机制和调控提供了有价值的新见解。
