诱导多能胚胎干细胞的生成：方法学章节
Generating primed pluripotent epiblast stem cells: A methodology chapter.
机构信息
Department of Human Genetics, University of Michigan Medical School, Ann Arbor, MI, United States.
Department of Human Genetics, University of Michigan Medical School, Ann Arbor, MI, United States.
出版信息
Curr Top Dev Biol. 2020;138:139-174. doi: 10.1016/bs.ctdb.2020.01.005. Epub 2020 Feb 27.
Abstract
At least two distinct pluripotent states, referred to as naïve and primed, define the early mammalian embryo. In the mouse, the pluripotent epiblast cells in the pre/peri-implantation embryo are the source of naïve embryonic stem cells (ESCs). After the embryo implants, the epiblast lineage generates a restricted or primed population of stem cells, referred to as epiblast stem cells (EpiSCs). ESCs can be cultured in EpiSC media to generate epiblast-like cells (EpiLCs). The differentiation of naive ESCs into primed EpiLCs permits insights into the development and differentiation of the pluripotent epiblast lineage. This chapter describes the generation and characterization of EpiSCs as well as EpiLCs.
摘要
至少有两种不同的多能状态，称为原始态和诱导态，定义了早期哺乳动物胚胎。在小鼠中，植入前/植入期胚胎中的多能上胚层细胞是原始态胚胎干细胞（ESCs）的来源。胚胎植入后，上胚层谱系产生了一种受限的或诱导的干细胞群体，称为上胚层干细胞（EpiSCs）。ESCs 可以在 EpiSC 培养基中培养，生成类上胚层细胞（EpiLCs）。将原始态 ESCs 分化为诱导态 EpiLCs 可以深入了解多能上胚层谱系的发育和分化。本章描述了 EpiSCs 和 EpiLCs 的生成和鉴定。
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