Posgrado en Ciencias Biológicas, Universidad Nacional Autónoma de México, A.P 70-153, Ciudad de México, C.P. 04510, Mexico; Instituto de Biología, Universidad Nacional Autónoma de México, A.P. 70-233, Ciudad de México, C.P. 04510, Mexico; Programa de Investigación de Producción de Biomoléculas, Departamento de Biología Molecular y Biotecnología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, AP. 70-228, Ciudad de México, C.P. 04510, Mexico.
Programa de Investigación de Producción de Biomoléculas, Departamento de Biología Molecular y Biotecnología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, AP. 70-228, Ciudad de México, C.P. 04510, Mexico.
Fungal Biol. 2020 Mar-Apr;124(3-4):205-218. doi: 10.1016/j.funbio.2020.02.003. Epub 2020 Feb 21.
In order to increase survival rates of greenhouse seedlings destined for restoration and conservation programs, successful mycorrhization of the seedlings is necessary. To reforest forest ecosystems, host trees must be inoculated with ectomycorrhizal fungi and, in order to guarantee a sufficient supply of ectomycorrhizal inoculum, it is necessary to develop technologies for the mass production of ectomycorrhizal fungi mycelia. We selected the ectomycorrhizal fungus Laccaria trichodermophora, due to its ecological traits and feasible mycelia production in asymbiotic conditions. Here, we report the field sampling of genetic resources, as well as the highly productive nutritional media and cultivation parameters in solid cultures. Furthermore, in order to achieve high mycelial production, we used strain screening and evaluated pH, carbon source concentration, and culture conditions of submerged cultures in normal and baffled shake flasks. The higher productivity culture conditions in shake flasks were selected for evaluation in a pneumatic bioreactor, using modified BAF media with a 10 g/L glucose, pH 5.5, 25 °C, and a volumetric oxygen transfer coefficient (Ka) of 36 h. Under those conditions less biomass (12-37 %) was produced in the pneumatic bioreactor compared with the baffled shake flasks. This approach shows that L. trichodermophora can generate a large biomass concentration and constitute the biotechnological foundation of its mycelia mass production.
为了提高用于恢复和保护计划的温室幼苗的存活率，必须成功地对幼苗进行菌根化。为了重新造林森林生态系统，宿主树木必须接种外生菌根真菌，并且为了保证外生菌根接种体的充足供应，有必要开发大规模生产外生菌根真菌菌丝体的技术。由于其生态特征和在非共生条件下可行的菌丝体生产，我们选择了外生菌根真菌 Tricholoma matsutake。在这里，我们报告了遗传资源的野外采样，以及在固体培养中高生产力的营养培养基和培养参数。此外，为了实现高菌丝体产量，我们使用了菌株筛选，并评估了 pH 值、碳源浓度以及普通和挡板摇瓶中的浸没培养条件。在气动生物反应器中，使用改良的 BAF 培养基（含 10g/L 葡萄糖、pH 值 5.5、25°C 和体积氧传递系数（Ka）为 36h），选择了摇瓶中生产力更高的培养条件进行评估。在这些条件下，与挡板摇瓶相比，气动生物反应器中产生的生物量（12-37%）较少。这种方法表明 Tricholoma matsutake 可以产生大量的生物质浓度，并构成其菌丝体大规模生产的生物技术基础。
