Masters student, Department of Dental Materials and Prosthodontics, School of Dentistry, São Paulo State University (UNESP), Araraquara, Brazil.
Doctoral student, Department of Dental Materials and Prosthodontics, School of Dentistry, São Paulo State University (UNESP), Araraquara, Brazil.
J Prosthet Dent. 2020 Jul;124(1):122.e1-122.e9. doi: 10.1016/j.prosdent.2020.01.030. Epub 2020 Mar 25.
If the components in the acrylic resins used to fabricate interim crows are cytotoxic, they can interfere with the integrity of the adjacent periodontal tissue and the dentin-pulp complex.
The purpose of this in vitro study was to assess the cytotoxicity of resin-based materials used to prepare interim crowns.
The following materials were used in this study: CAR, conventional acrylic resin powder and liquid; BR, bis-acrylic resin; and PAR, pressed acrylic resin of the CAD-CAM type. Glass disks were used as the control (Co). Oral epithelial cells (NOK) were seeded on glass disks and standardized disks prepared with the resins under study. After incubation for 24 hours, the cells were analyzed for viability (Alamar Blue and Live or Dead), adhesion, and morphology (SEM and fluorescence), as well as epidermal growth factor synthesis (EGF-ELISA). The surface roughness (Ra) of test specimens was evaluated under a confocal microscope. The data were submitted to ANOVA and the Tukey HSD statistical tests (α=.05).
The highest Ra value was observed in BR in comparison with CAR, PAR, and Co (P<.05). The highest viability, adhesion, and EGF synthesis values were determined for the cells in contact with PAR (P<.001).
The computer-aided design and computer-aided manufacturing (CAD-CAM)-type resin favored adhesion, metabolism, and epithelial cell proliferation, and it was therefore considered cytocompatible.
如果用于制作临时牙冠的丙烯酸树脂中的成分具有细胞毒性，它们可能会干扰牙周组织和牙本质牙髓复合体的完整性。
本体外研究的目的是评估用于制备临时牙冠的树脂基材料的细胞毒性。
本研究使用了以下材料：CAR，传统的丙烯酸树脂粉和液体；BR，双丙烯酸树脂；以及 PAR，CAD-CAM 型压克力树脂。玻璃盘被用作对照（Co）。将口腔上皮细胞（NOK）接种在玻璃盘和用研究中的树脂制备的标准盘上。孵育 24 小时后，分析细胞活力（Alamar Blue 和 Live or Dead）、粘附和形态（SEM 和荧光），以及表皮生长因子合成（EGF-ELISA）。在共聚焦显微镜下评估测试样本的表面粗糙度（Ra）。将数据提交给方差分析和 Tukey HSD 统计检验（α=.05）。
与 CAR、PAR 和 Co 相比，BR 的 Ra 值最高（P<.05）。与 PAR 接触的细胞表现出最高的活力、粘附和 EGF 合成值（P<.001）。
计算机辅助设计和计算机辅助制造（CAD-CAM）型树脂有利于粘附、代谢和上皮细胞增殖，因此被认为是细胞相容的。
