Mandel M J, Lim H W
Dermatology Service, New York Veterans Administration Medical Center, New York.
Photodermatol. 1988 Oct;5(5):211-7.
Exposure of highly purified rat serosal mast cells to UVA (34-340 kJ/m2), in the presence or absence of 8-methoxypsoralen (100 ng/ml), or to UVB (160-640 J/m2), resulted in dose-dependent releases of mast cell preformed mediators, as measured by the release of radioactivity from 3H-serotonin-labeled cells. The net release ranged from 2.3% to 14.2%. The above treatments had no effect on mediator release induced by subsequent incubation with calcium ionophore A23187 (0.4 and 4.0 mumol/l) or with compound 48/80 (1.0 microgram/ml), with the exception that exposure to UVB did suppress the release induced by the latter. These results indicate that under clinically relevant conditions, the direct effect of in vitro ultraviolet radiation on mast cells did not alter the ability of these cells to respond to subsequent stimulation with secretagogues.
将高度纯化的大鼠浆膜肥大细胞暴露于UVA（34 - 340 kJ/m²），无论有无8 - 甲氧基补骨脂素（100 ng/ml）存在，或暴露于UVB（160 - 640 J/m²），都会导致肥大细胞预先形成的介质呈剂量依赖性释放，这通过从³H - 5 - 羟色胺标记的细胞中释放放射性来测量。净释放率在2.3%至14.2%之间。上述处理对随后用钙离子载体A23187（0.4和4.0 μmol/l）或化合物48/80（1.0 μg/ml）孵育诱导的介质释放没有影响，不过暴露于UVB确实抑制了后者诱导的释放。这些结果表明，在临床相关条件下，体外紫外线辐射对肥大细胞的直接作用并未改变这些细胞对随后促分泌剂刺激的反应能力。
