Amano H, Kurosawa M, Miyachi Y
Department of Dermatology, Gunma University School of Medicine, Maebashi, Japan.
Clin Exp Allergy. 1997 Aug;27(8):966-71.
Ultraviolet light irradiation has been shown to suppress immunological reactions of irradiated individuals, however, less attention has been paid to the effects on neurogenic inflammation.
We have investigated the effect of ultraviolet light B (UVB) irradiation on substance P (SP)-induced histamine release from rat mast cells.
Rat peritoneal mast cells were treated with UVB irradiation and challenged by SP. Histamine release from the cells and intracellular calcium concentrations ([Ca2+]i) in the cells were measured.
UVB irradiation at doses used in the present study did not induce histamine release from mast cells. UVB irradiation at doses from 25 to 200 mJ/cm2 inhibited 10(-5) mol/L SP-induced histamine release in a dose-dependent manner. On the other hand, SP-induced increase of [Ca2+]i was inhibited by UVB irradiation only at doses of 100 and 200 mJ/cm2.
These data suggest that UVB irradiation inhibits histamine release from SP-activated rat peritoneal mast cells partially through the suppression of an increase in [Ca2+]i.
紫外线照射已被证明可抑制受照射个体的免疫反应，然而，对其对神经源性炎症的影响关注较少。
我们研究了紫外线B（UVB）照射对P物质（SP）诱导的大鼠肥大细胞组胺释放的影响。
用UVB照射大鼠腹腔肥大细胞，并用SP进行刺激。测量细胞中的组胺释放和细胞内钙浓度（[Ca2+]i）。
本研究中使用的剂量的UVB照射未诱导肥大细胞释放组胺。25至200 mJ/cm2剂量的UVB照射以剂量依赖方式抑制10(-5)mol/L SP诱导的组胺释放。另一方面，仅在100和200 mJ/cm2剂量下，UVB照射抑制了SP诱导的[Ca2+]i增加。
这些数据表明，UVB照射通过抑制[Ca2+]i增加而部分抑制SP激活的大鼠腹腔肥大细胞释放组胺。
