Diukman R, Zeigler M, Bach G
Department of Human Genetics, Hadassah University Hospital, Jerusalem, Israel.
Prenat Diagn. 1988 Sep;8(7):531-7. doi: 10.1002/pd.1970080708.
The relatively high activity of arylsulphatase C (ASC) in the placenta is a potential risk for the misdiagnosis of arylsulphatase A (ASA) or arylsulphatase B (ASB) deficiency in chorionic villus sampling when assayed by synthetic substrates. A clear distinction between these enzymes can be achieved in either the direct villi or the cultured villi cells. Interestingly, the activity of ASC differed significantly in cultured villi cells when prepared by two different methods, namely, minced villi versus treatment with trypsin and collagenase, while ASA and ASB were not affected by these treatments. Whether ASC was directly affected by one of these treatments or whether a selection of cells with different ASC levels was achieved is not yet clear, but this phenomenon clearly indicates the importance of precise definition of CVS preparations to correlate with the enzyme activity data.
胎盘组织中芳基硫酸酯酶C（ASC）活性相对较高，这在通过合成底物检测时，会增加绒毛取样中芳基硫酸酯酶A（ASA）或芳基硫酸酯酶B（ASB）缺乏症误诊的潜在风险。在直接绒毛或培养的绒毛细胞中，可以明确区分这些酶。有趣的是，通过两种不同方法制备培养的绒毛细胞时，即绒毛切碎法与胰蛋白酶和胶原酶处理法，ASC的活性存在显著差异，而ASA和ASB不受这些处理的影响。目前尚不清楚ASC是否直接受到其中一种处理的影响，还是实现了不同ASC水平细胞的选择，但这一现象清楚地表明了精确定义绒毛取样制备方法以关联酶活性数据的重要性。
