自由对流 PCR：从原理研究到商业应用——批判性综述。
Free convective PCR: From principle study to commercial applications-A critical review.
机构信息
Institute of Microfluidic Chip Development in Biomedical Engineering, College of Information Science and Technology, Beijing University of Chemical Technology, Beijing, 100029, China.
National Institute of Diagnostics and Vaccine Development in Infectious Diseases, Xiamen University, Xiamen, 361005, China.
出版信息
Anal Chim Acta. 2020 Apr 29;1108:177-197. doi: 10.1016/j.aca.2020.01.069. Epub 2020 Jan 31.
Polymerase chain reaction (PCR) is an extremely important tool for molecular diagnosis, as it can specifically amplify nucleic acid templates for sensitive detection. As another division of PCR, free convective PCR was invented in 2001, which can be performed in a capillary tube pseudo-isothermally within a significantly short time. Convective PCR thermal cycling is implemented by inducing thermal convection inside the capillary tube, which stratifies the reaction into spatially separate and stable melting, annealing, and extension zones created by the temperature gradient. Convective PCR is a promising tool that can be used for nucleic acid diagnosis as a point-of-care test (POCT) due to the significantly simplified heating strategy, reduced cost, and shortened detection time without sacrificing sensitivity and accuracy. Here, we review the history of free convective PCR from its invention to development and its commercial applications.
聚合酶链反应（PCR）是分子诊断的重要工具，因为它可以特异性地扩增核酸模板，进行敏感检测。作为 PCR 的另一个分支，自由对流 PCR 于 2001 年发明，可以在毛细管内准恒温下在极短的时间内完成。对流 PCR 热循环通过在毛细管内诱导热对流来实现，该对流将反应分层为空间上分开且稳定的熔融、退火和延伸区，由温度梯度产生。对流 PCR 是一种很有前途的工具，可作为即时检测（POCT）用于核酸诊断，因为其加热策略大大简化，成本降低，检测时间缩短，而不会牺牲灵敏度和准确性。在这里，我们回顾了自由对流 PCR 从发明到发展及其商业应用的历史。
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