Dariush N, Fisher C W, Steggles A W
Department of Biochemistry and Molecular Pathology, Northeastern Ohio Universities College of Medicine, Rootstown 44272.
Protein Seq Data Anal. 1988;1(5):351-3.
Using a synthetic 26 base pair (bp) oligonucleotide, we have screened a rabbit liver cDNA library in lambda gt11 and isolated a 1000 bp DNA clone corresponding to cytochrome b5 mRNA. The clone contains the complete coding region and long 5' and 3' non-translated regions. The derived amino acid sequence (sequence; see text) agrees with published sequences, and confirms that the amino acids 62 and 104 are asparagine and aspartic acid, respectively.
我们使用一条26个碱基对（bp）的合成寡核苷酸，筛选了λgt11载体中的兔肝cDNA文库，并分离出一个与细胞色素b5 mRNA对应的1000 bp DNA克隆。该克隆包含完整的编码区以及长的5'和3'非翻译区。推导的氨基酸序列（序列；见正文）与已发表的序列一致，并证实氨基酸62和104分别为天冬酰胺和天冬氨酸。
