Zhang H, Somerville C
Genetics Program, Michigan State University, East Lansing 48824.
Arch Biochem Biophys. 1988 Jul;264(1):343-7. doi: 10.1016/0003-9861(88)90603-0.
A cDNA clone encoding the chicken liver cytochrome b5 was isolated by probing a library with synthetic oligonucleotides based on a partial amino acid sequence of the protein. Determination of the DNA sequence indicated a 414-nucleotide open reading frame which encodes a 138-amino acid residue polypeptide. The open reading frame contains 6 amino acids at the amino terminus which were not present on any of the cytochrome b5 polypeptides for which the amino acid sequence has been determined directly, suggesting that the protein is proteolytically processed to the mature form. The results of genomic Southern analysis were consistent with the presence of two structurally different genes in the chicken genome, raising the possibility that the soluble and membrane-bound forms of the protein are the products of separate genes.
通过用基于该蛋白质部分氨基酸序列的合成寡核苷酸探测文库，分离出了编码鸡肝细胞色素b5的cDNA克隆。DNA序列测定表明有一个414个核苷酸的开放阅读框，其编码一个138个氨基酸残基的多肽。该开放阅读框在氨基末端含有6个氨基酸，这些氨基酸在任何已直接测定氨基酸序列的细胞色素b5多肽上都不存在，这表明该蛋白质经过蛋白水解加工成为成熟形式。基因组Southern分析结果与鸡基因组中存在两个结构不同的基因一致，这增加了该蛋白质的可溶性和膜结合形式是不同基因产物的可能性。
