Institute and Outpatient Clinic of Occupational, Social and Environmental Medicine, Friedrich-Alexander Universität Erlangen-Nürnberg, Henkestraße 9-11, 91054 Erlangen, Germany.
Institute and Outpatient Clinic of Occupational, Social and Environmental Medicine, Friedrich-Alexander Universität Erlangen-Nürnberg, Henkestraße 9-11, 91054 Erlangen, Germany.
J Chromatogr B Analyt Technol Biomed Life Sci. 2020 May 1;1144:122071. doi: 10.1016/j.jchromb.2020.122071. Epub 2020 Mar 19.
2-(2H-Benzotriazol-2-yl)-4,6-di-tert-pentylphenol (UV 328) is an ultraviolet light (UV) absorber which prolongs the stability of plastics and other organic compounds towards UV radiation. Therefore, it is frequently used as a preserving additive and may result in an exposure of consumers. Thus, an analytical method using dispersive liquid-liquid microextraction and subsequent gas chromatography tandem mass spectrometry analysis with advanced electron ionization was developed for a human biomonitoring of UV 328 exposure, which enabled the determination of UV 328 and six of its metabolites in human urine. Sample preparation and derivatization were optimized. Baseline separation of the analytes was assured by the application of a suitable temperature program. The validation resulted in limits of detection of 0.1 µg/L for all analytes, variation coefficients from 1 to 12% for precision in series and from 5 to 12% for interday precision. Furthermore, relative recovery rates between 80 and 120% were determined. Moreover, the procedure was successfully applied to urine samples of a volunteer exposed to UV 328. The method showed excellent sensitivity, repeatability and robustness for all parameters and may be applicable for studies to elucidate the metabolism and kinetics of the investigated UV absorber and for monitoring of individuals with specific exposure to UV 328.
2-(2H-苯并三唑-2-基)-4,6-二叔戊基苯酚（UV 328）是一种紫外线（UV）吸收剂，可延长塑料和其他有机化合物对 UV 辐射的稳定性。因此，它经常被用作防腐剂，可能会导致消费者暴露。因此，开发了一种使用分散液 - 液微萃取和随后的气相色谱串联质谱分析与先进的电子电离的分析方法，用于人体对 UV 328 暴露的生物监测，该方法能够测定人尿中 UV 328 和其六种代谢物。优化了样品制备和衍生化。通过应用合适的温度程序，确保了分析物的基线分离。验证结果表明，所有分析物的检出限均为 0.1μg/L，精密度的变异系数为 1%至 12%，日内精密度的变异系数为 5%至 12%。此外，还确定了 80%至 120%的相对回收率。此外，该程序已成功应用于暴露于 UV 328 的志愿者的尿液样本。该方法对所有参数均表现出优异的灵敏度、重复性和稳健性，可适用于阐明所研究的紫外线吸收剂的代谢和动力学以及监测特定暴露于 UV 328 的个体的研究。
