Denghel Heike, Göen Thomas
Institute and Outpatient Clinic of Occupational, Social and Environmental Medicine, Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen, Germany.
Institute and Outpatient Clinic of Occupational, Social and Environmental Medicine, Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen, Germany.
Talanta. 2021 Feb 1;223(Pt 1):121699. doi: 10.1016/j.talanta.2020.121699. Epub 2020 Sep 28.
2-(2H-Benzotriazol-2-yl)-4,6-di-tert-pentylphenol (UV 328; CAS: 25973-55-1) is a benzotriazole ultraviolet light (BUV) absorber which is applied to plastics and other organic substances to prevent discoloration and enhance product stability. Therefore, UV 328 is frequently used as a plastic additive and may lead to an exposure of consumers. For a reliable assessment of UV 328 metabolism, an analytical method applying dispersive liquid-liquid microextraction (DLLME) followed by gas chromatography tandem mass spectrometry and advanced electron ionization was developed which allows the determination of UV 328 and six of its metabolites in human whole blood. Sample preparation was optimized with respect to DLLME parameters. A critical aspect of the procedure was the application of spiked human blood for calibration, which proved to be essential for achieving accurate results. Validation of the method resulted in limits of detection of 0.1 μg/L for all analytes. Variation coefficients ranged from 2 to 9% for intraday precision and from 3 to 11% for interday precision. Furthermore, relative recovery rates between 80 and 100% were calculated. Afterwards, the procedure was successfully applied to blood samples collected from a volunteer orally exposed to a single dose of UV 328. The method proved to be highly sensitive, repeatable and robust for all compounds and may further be used for studies to elucidate the human metabolism and kinetics of UV 328 and for biomonitoring of specific, environmental and occupational exposure to this UV stabilizer.
2-(2H-苯并三唑-2-基)-4,6-二叔戊基苯酚（UV 328；CAS：25973-55-1）是一种苯并三唑类紫外线（BUV）吸收剂，用于塑料和其他有机物质，以防止变色并提高产品稳定性。因此，UV 328经常用作塑料添加剂，可能导致消费者接触。为了可靠评估UV 328的代谢情况，开发了一种分析方法，该方法采用分散液液微萃取（DLLME），随后进行气相色谱串联质谱分析和先进的电子电离，可测定人全血中的UV 328及其六种代谢物。针对DLLME参数对样品制备进行了优化。该程序的一个关键方面是使用加标的人血进行校准，这被证明对获得准确结果至关重要。该方法的验证结果表明，所有分析物的检测限均为0.1μg/L。日内精密度的变异系数范围为2%至9%，日间精密度的变异系数范围为3%至11%。此外，计算出的相对回收率在80%至100%之间。之后，该程序成功应用于从一名口服单剂量UV 328的志愿者采集的血样。该方法对所有化合物均具有高度敏感性、可重复性和稳健性，可进一步用于阐明UV 328的人体代谢和动力学的研究，以及对该紫外线稳定剂的特定、环境和职业暴露的生物监测。
