Unalan-Altintop Tugce, Karagoz Alper, Hazirolan Gulsen
1Department of Medical Microbiology, Hacettepe University, Faculty of Medicine, Ankara, Turkey.
2Department of Microbiology, Usak University, Faculty of Molecular Biology and Genetics, Usak, Turkey.
Acta Microbiol Immunol Hung. 2020 Mar 30;67(4):258-260. doi: 10.1556/030.2020.01039.
MALDI-TOF MS provides fast, easy to perform and cost-effective diagnosis in clinical microbiology laboratories, however in some cases results of MALDI-TOF MS should be confirmed with additional tests. This confirmation is especially important for causes of life-threatening infections like Neisseria meningitidis. In our laboratory, three isolates were identified as N. meningitidis by Bruker MALDI Biotyper (BD, USA) between April 2018 and March 2019 from clinical specimens of blood, sputum, and urine. 16S rRNA sequencing was performed for further investigation. Two of the isolates were identified as Neisseria subflava and only one was confirmed as N. meningitidis by sequencing. These results show that MALDI-TOF MS is not always reliable in the diagnosis of N. meningitidis and clinical microbiologists should confirm these results with additional tests. Also, clinical correlations should be determined. Accurate identification of this microorganism is very important because of the necessity of prophylactic antimicrobial usage and biosafety precautions. Enlarged databases of Neisseria species are needed to overcome this problem.
基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）在临床微生物实验室中可提供快速、易于操作且经济高效的诊断，然而在某些情况下，MALDI-TOF MS的结果应通过额外的检测进行确认。这种确认对于像脑膜炎奈瑟菌这样危及生命的感染病因尤为重要。在我们实验室，2018年4月至2019年3月期间，从血液、痰液和尿液的临床标本中，有三株分离菌通过布鲁克MALDI Biotyper（美国BD公司）被鉴定为脑膜炎奈瑟菌。为进一步调查进行了16S rRNA测序。其中两株分离菌被鉴定为微黄奈瑟菌，只有一株通过测序被确认为脑膜炎奈瑟菌。这些结果表明，MALDI-TOF MS在脑膜炎奈瑟菌的诊断中并不总是可靠的，临床微生物学家应通过额外的检测来确认这些结果。此外，还应确定临床相关性。由于预防性使用抗菌药物和采取生物安全预防措施的必要性，准确鉴定这种微生物非常重要。需要扩大奈瑟菌属的数据库来解决这个问题。
