Département des Agents Infectieux, Hôpitaux Universitaires Saint-Louis, Lariboisière, Fernand-Widal, APHP, Paris, France.
Infection, Antimicrobiens, Modélisation, Evolution (IAME), UMR1137, INSERM, Université Paris Diderot, Paris, France.
Eur J Clin Microbiol Infect Dis. 2018 Dec;37(12):2273-2277. doi: 10.1007/s10096-018-3368-6. Epub 2018 Sep 3.
Through the past decade, MALDI-TOF MS has been recognized as a fast and robust tool for identification of most bacteria in clinical microbiology. However, the accuracy of this method to identify Neisseria species is still debated, and few data are available about commensal Neisseria species identification. In this study, we assessed two MALDI-TOF MS systems (Bruker Biotyper and Andromas) for the identification of 88, 18, and 29 isolates of Neisseria gonorrhoeae, Neisseria meningitidis, and commensal Neisseria species, respectively. All 88 isolates of N. gonorrhoeae were correctly identified using both systems, and most N. meningitidis and commensal Neisseria species were well identified: only 1/18 isolates of N. meningitidis was misidentified using Bruker Biotyper, and 1 isolate of Neisseria polysaccharea was misidentified as N. meningitidis using both systems. These results strengthen the possibility to use MALDI-TOF MS as a single method for Neisseria identification in routine, with excellent performance for N. gonorrhoeae identification. However, results should be interpreted prudently for N. meningitdis and commensal Neisseria species when isolated from genital and oropharyngeal samples where these both species can coexist.
在过去的十年中，MALDI-TOF MS 已被公认为一种快速而强大的工具，可用于鉴定临床微生物学中的大多数细菌。然而，这种方法对奈瑟菌属物种的鉴定准确性仍存在争议，关于共生奈瑟菌属物种的鉴定数据很少。在这项研究中，我们评估了两种 MALDI-TOF MS 系统（布鲁克 Biotyper 和 Andromas），分别用于鉴定 88 株淋病奈瑟菌、18 株脑膜炎奈瑟菌和 29 株共生奈瑟菌属。使用两种系统均正确鉴定了所有 88 株淋病奈瑟菌，大多数脑膜炎奈瑟菌和共生奈瑟菌属也得到了很好的鉴定：仅 18 株脑膜炎奈瑟菌中有 1 株被布鲁克 Biotyper 误鉴定，1 株多糖奈瑟菌被两种系统误鉴定为脑膜炎奈瑟菌。这些结果增强了 MALDI-TOF MS 作为常规奈瑟菌鉴定的单一方法的可能性，对淋病奈瑟菌的鉴定具有出色的性能。然而，当从生殖道和口咽样本中分离出这些两种共存的物种时，应谨慎解释脑膜炎奈瑟菌和共生奈瑟菌属的结果。
