Center for Biologics Evaluation and Research, U.S. Food and Drug Administration, 10903 New Hampshire Avenue, Silver Spring, MD 20993, USA.
Bioinformatics and Systems Biology, Justus-Liebig-University Giessen, Heinrich Buff Ring 58, 35392, Giessen, Germany.
Int J Syst Evol Microbiol. 2020 May;70(5):3037-3048. doi: 10.1099/ijsem.0.004127.
Two independent strains of a species (ES3154-GLU and ES2714_GLU) were isolated from the oral cavity of northern elephant seals () that were admitted to The Marine Mammal Centre facilities in California, USA. The strains were isolated from oral swabs by cultivation in PPLO broth supplemented with serum, penicillin and colistin in anaerobic conditions. The strains were Gram-negative, pleomorphic, indole-, oxidase- and catalase-negative, non-spore-forming, non-motile rods/coccobacilli in short chains. The 16S rRNA gene sequence of these strains shared 94.42 % nucleotide similarity with AVG 2115 but demonstrated ≤86.00-92.50 % nucleotide similarity to the 16S rRNA genes of other species of the family . The genome was sequenced for strain ES3154-GLU. Average nucleotide identity values between strain ES3154-GLU and 15 type strain genomes from the family ranged from 66.74 % vs. to 73.35 % vs. . The whole genome phylogeny revealed that the novel species was most closely related to AVG 2115. This relationship was also confirmed by nucleotide similarity and multilocus phylogenetic analyses employing various housekeeping genes (partial 23S rRNA, , , , , , and genes). Chemotaxonomic and phenotypical features of strain ES3154-GLU were in congruence with closely related members of the family , represented by similar enzyme profiles and fatty acid patterns. MALDI-TOF MS analysis was capable to clearly discriminate strain ES3154-GLU from all currently described taxa of the family . Based on these data, we propose a novel species of the genus , for which the name sp. nov. is proposed with the type strain ES3154-GLU (=DSM 109740=CCUG 73653=ATCC TSD-189=NCTC 14411) and one representative strain ES2714_GLU. The G+C content is 26.82 %, genome size is 1 356 983 bp.
从美国加利福尼亚州海洋哺乳动物中心收治的北象海豹口腔中分离到两种独立的 种菌株（ES3154-GLU 和 ES2714_GLU）。这些菌株是从口腔拭子中通过在补充有血清、青霉素和多粘菌素的 PPLO 肉汤中进行厌氧培养分离出来的。这些菌株是革兰氏阴性、多形性、吲哚阴性、氧化酶和触酶阴性、无孢子形成、非运动的杆状/球杆菌，短链。这些菌株的 16S rRNA 基因序列与 AVG 2115 共享 94.42%的核苷酸相似性，但与家族中其他种的 16S rRNA 基因显示≤86.00-92.50%的核苷酸相似性。对 ES3154-GLU 菌株进行了基因组测序。ES3154-GLU 菌株与家族 15 个模式菌株基因组之间的平均核苷酸同一性值在 66.74%至 73.35%之间，范围为 66.74%至 73.35%。全基因组系统发育分析表明，该新种与 AVG 2115 最为密切相关。这一关系也通过使用各种管家基因（部分 23S rRNA、、、、、、和 基因）的核苷酸相似性和多基因系统发育分析得到了证实。ES3154-GLU 菌株的化学生物和表型特征与该家族的密切相关成员一致，表现出相似的酶谱和脂肪酸模式。MALDI-TOF MS 分析能够将 ES3154-GLU 菌株与该家族中所有已描述的分类群清楚地区分开来。基于这些数据，我们提出了一个新的 属种，建议使用 ES3154-GLU 菌株（=DSM 109740=CCUG 73653=ATCC TSD-189=NCTC 14411）和代表菌株 ES2714_GLU 作为该新种的模式菌株。该菌株的 G+C 含量为 26.82%，基因组大小为 1356983bp。
