Department of Tissue Engineering and Applied Cell Sciences, Faculty of Medicine, Semnan University of Medical Sciences, Semnan, Iran.
Department of Tissue Engineering, School of Advanced Technologies, Shahrekord University of Medical Sciences, Shahrekord, Iran.
Tissue Cell. 2020 Apr;63:101324. doi: 10.1016/j.tice.2019.101324. Epub 2019 Dec 16.
Three-dimensional (3D) cell culture is more similar to in vivo studies and suitable for studies of interactions between cells and extracellular matrix. CD44 is a cell surface receptor that can relate with the extracellular matrix molecules. CD44 in gastric cancer (GC) is a metastatic and drug resistance marker. In this study the quantity of CD44 cells in MKN-45 cell line in response to half maximal inhibitory concentration (IC) dose of Docetaxel (DOC) was measured in 2D and 3D cultures. MKN-45 cell line was cultured in 2D and 3D environments. For 3D culture, rat gastric tissue was separated and decellularized and MKN-45 cells were injected and cultured in the prepared matrix. The frequency of CD44 cells in 2D and 3D cultures were analyzed before and after treatment with IC of DOC by flow cytometry and immunohistochemistry. Despite different environmental conditions, The frequency of CD44 cells increased significantly in 2D and 3D environments after treatment with IC of DOC (P < 0.05). Given the advantages of 3D, this environment seems more appropriate for study about CD44 cells and drug resistance in GC.
三维（3D）细胞培养更类似于体内研究，适合研究细胞与细胞外基质之间的相互作用。CD44 是一种细胞表面受体，可与细胞外基质分子相关联。胃癌（GC）中的 CD44 是转移和耐药性的标志物。在这项研究中，在 2D 和 3D 培养中测量了 MKN-45 细胞系对多西紫杉醇（DOC）半最大抑制浓度（IC）剂量的反应中 CD44 细胞的数量。MKN-45 细胞系在 2D 和 3D 环境中培养。对于 3D 培养，分离并脱细胞大鼠胃组织，并将 MKN-45 细胞注入并在制备的基质中培养。通过流式细胞术和免疫组织化学分析在 2D 和 3D 培养中用 IC DOC 处理前后 CD44 细胞的频率。尽管环境条件不同，但在 2D 和 3D 环境中用 IC DOC 处理后，CD44 细胞的频率显着增加（P <0.05）。考虑到 3D 的优势，这种环境似乎更适合研究 GC 中的 CD44 细胞和耐药性。
