Khazaei Mozafar, Khazaei Mohammad Rasool, Kadivarian Sepideh, Hama Shahid Muhsin, Hussein Hevar Hassan, Haghighimanesh Hojjat, Rezakhani Leila
Fertility and Infertility Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran.
Department of Tissue Engineering, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran.
Regen Ther. 2024 Oct 7;26:867-878. doi: 10.1016/j.reth.2024.09.017. eCollection 2024 Jun.
Tissue regeneration in many skin defects is progressing with new treatments in recent years. Tissue engineering with the use of scaffolds offers more versatile and faster solutions in treatment. Extracellular matrix (ECM) and its three-dimensional (3D) network structure as a biological bond by imitating the tissue microstructure has been used for tissue repair, which can answer many existing challenges. Vitamin A, which comes in several forms such as retinols, retinals, and retinoic acids, is a necessary vitamin that is crucial for wound healing. In this research, sheep kidney capsule tissue decellularized with sodium dodecyl sulfate (SDS) containing different doses of vitamin A has been used as an ECM in skin tissue engineering. The above scaffold was evaluated in terms of properties such as biocompatibility, analysis of mechanical properties, attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR), hydrophilicity, antibacterial, and cell adhesion. The findings reported suitable properties for wound dressing, especially at a dose of 15,000 U/ml vitamin A for this scaffold. Then, the above scaffold was evaluated on the full-thickness wound model in rat, which showed good wound contraction, and increased VEGF factor. It showed a decrease in IL-1β level. Therefore, the use of the above-mentioned decellularized scaffold in combination with medicinal agents effective in wound healing can be introduced for further pre-clinical studies.
近年来，随着新疗法的出现，许多皮肤缺损的组织再生取得了进展。利用支架的组织工程学在治疗中提供了更多通用且快速的解决方案。细胞外基质（ECM）及其三维（3D）网络结构通过模仿组织微观结构作为一种生物粘合剂已被用于组织修复，这可以应对许多现有挑战。维生素A有多种形式，如视黄醇、视黄醛和视黄酸，是一种对伤口愈合至关重要的必需维生素。在本研究中，用含有不同剂量维生素A的十二烷基硫酸钠（SDS）去细胞化的羊肾包膜组织已被用作皮肤组织工程中的细胞外基质。上述支架在生物相容性、力学性能分析、衰减全反射傅里叶变换红外光谱（ATR-FTIR）、亲水性、抗菌性和细胞粘附等性能方面进行了评估。研究结果表明该支架具有适合伤口敷料的性能，尤其是对于该支架维生素A剂量为15,000 U/ml时。然后，在大鼠全层伤口模型上对上述支架进行了评估，结果显示伤口收缩良好，血管内皮生长因子（VEGF）因子增加。白细胞介素-1β（IL-1β）水平降低。因此，上述去细胞化支架与对伤口愈合有效的药物联合使用可引入进一步的临床前研究。
