Tengzhou Central People's Hospital, Department of Hand Surgery, Tengzhou, 277500 Zaozhuang, Shandong province, China.
Western Pharmacy, Wangkai Hospital, Tengzhou, 277500 Zaozhuang, Shandong province, China.
Tissue Cell. 2020 Apr;63:101331. doi: 10.1016/j.tice.2020.101331. Epub 2020 Jan 9.
The purpose of this exploration was to detect the biological effects of miR-10b/FAM46C pair on osteosarcoma (OS) development. By accessing to the Gene Expression Omnibus (GEO) database, we achieved expressional profiles of miR-10b and FAM46C. Kaplan-Meier method was applied to determine the overall survival rates of OS patients. MiR-10b mimic/inhibitor were utilized to alter miR-10b expression. Overexpression of FAM46C was induced by pcDNA3.1-FAM46C. QRT-PCR and western blot were conducted to assess the expression levels. Cell counting kit-8 (CCK-8) and transwell assays were employed to evaluate the proliferative, invasive and migratory properties of OS cells. Pearson correlation analysis was performed to confirm the association between miR-10b and FAM46C. Dual-luciferase reporter assay was conducted to determine the target of miR-10b. The overall survival of OS patients was inversely correlated with miR-10b expression. MiR-10b was increased in OS compared with normal controls. Depletion of miR-10b attenuated the proliferation, invasion and migration of MG-63 cells. FAM46C was considered as a target gene of miR-10b and inversely related with miR-10b. Overexpression of FAM46C could inhibit cell growth, invasion and migration in OS; furthermore, it also can enforced the miR-10b inhibitor-induced effects on cell behaviors of OS cells. Down-regulation of miR-10b played a suppressive effect on the cell activity in OS cells, which provides a novel insight into the advance of OS therapeutic therapies.
本研究旨在探究 miR-10b/FAM46C 对骨肉瘤（OS）发展的生物学影响。我们通过访问基因表达综合数据库（GEO），获得了 miR-10b 和 FAM46C 的表达谱。Kaplan-Meier 方法用于确定 OS 患者的总生存率。利用 miR-10b 模拟物/抑制剂改变 miR-10b 的表达。通过 pcDNA3.1-FAM46C 诱导 FAM46C 的过表达。利用 QRT-PCR 和 Western blot 检测 miR-10b 和 FAM46C 的表达水平。细胞计数试剂盒-8（CCK-8）和 Transwell 实验用于评估 OS 细胞的增殖、侵袭和迁移能力。采用 Pearson 相关性分析确认 miR-10b 与 FAM46C 之间的相关性。双荧光素酶报告基因实验确定 miR-10b 的靶基因。OS 患者的总生存率与 miR-10b 的表达呈负相关。与正常对照组相比，OS 中 miR-10b 表达增加。miR-10b 耗竭可减弱 MG-63 细胞的增殖、侵袭和迁移。FAM46C 被认为是 miR-10b 的靶基因，与 miR-10b 呈负相关。FAM46C 的过表达可抑制 OS 细胞的生长、侵袭和迁移；此外，它还能增强 OS 细胞中 miR-10b 抑制剂对细胞行为的影响。miR-10b 的下调对 OS 细胞的细胞活性具有抑制作用，为 OS 治疗提供了新的见解。
