Department of Life Sciences, University of Trieste, 34127 Trieste, Italy.
Department of Medical Biotechnologies, University of Siena, Siena, Italy; Department of Biology, University of Rome "Tor Vergata", Rome, Italy.
Int J Biol Macromol. 2020 Jul 15;155:315-323. doi: 10.1016/j.ijbiomac.2020.03.196. Epub 2020 Mar 26.
Klebsiella pneumoniae strain KPB-1 was isolated in early 2011 from the pleural fluid of an inpatient admitted at an Italian hospital. It was characterized to produce the KPC-3 carbapenemase and to belong to sequence type 512, a derivative of sequence type 258 clade II characterized by the cps-2 gene cluster. The K-antigen of K. pneumoniae KPB-1 was purified and its structure determined by using GLC-MS of appropriate carbohydrate derivatives and 1D and 2D NMR spectroscopy of the native polysaccharide. All the collected data demonstrated the following repeating unit for the K. pneumoniae KPB-1 capsular polysaccharide: The reactions catalysed by each glycosyltransferase in the cps-2 gene cluster were assigned on the basis of structural homology with other Klebsiella K antigens.
肺炎克雷伯菌菌株 KPB-1 于 2011 年初从一名入住意大利医院的住院患者的胸腔积液中分离出来。它的特点是产生 KPC-3 碳青霉烯酶，属于序列类型 512，是由 cps-2 基因簇特征的序列类型 258 分支 II 的衍生物。肺炎克雷伯菌 KPB-1 的 K 抗原被纯化，并通过适当的碳水化合物衍生物的 GLC-MS 和天然多糖的 1D 和 2D NMR 光谱确定其结构。所有收集的数据表明肺炎克雷伯菌 KPB-1 荚膜多糖的重复单元为：基于与其他肺炎克雷伯菌 K 抗原的结构同源性，确定了 cps-2 基因簇中每个糖基转移酶催化的反应。
