Alliance for Research on the Mediterranean and North Africa (ARENA), University of Tsukuba, Tsukuba 305-8577, Ibaraki, Japan.
R&D Center for Tailor-Made QOL, University of Tsukuba, Tsukuba 305-8550, Ibaraki, Japan.
Aging (Albany NY). 2020 Mar 29;12(6):5516-5538. doi: 10.18632/aging.102985.
Alzheimer's disease (AD) has become a major world health problem as the population ages. There is still no available treatment that can stop or reverse the progression of AD. Human amnion epithelial cells (hAECs), an alternative source for stem cells, have shown neuroprotective and neurorestorative potentials when transplanted . Besides, studies have suggested that stem cell priming with plant-derived bioactive compounds can enhance stem cell proliferation and differentiation and improve the disease-treating capability of stem cells. Verbenalin is an iridoid glucoside found in medicinal herbs of Verbenaceae family. In the present study, we have conducted microarray gene expression profiling of verbenalin-treated hAECs to explore its therapeutic potential for AD. Gene set enrichment analysis revealed verbenalin treatment significantly enriched AD-associated gene sets. Genes associated with lysosomal dysfunction, pathologic angiogenesis, pathologic protein aggregation, circadian rhythm, age-related neurometabolism, and neurogenesis were differentially expressed in the verbenalin-treated hAECs compared to control cells. Additionally, the neuroprotective effect of verbenalin was confirmed against amyloid beta-induced neurotoxicity in human neuroblastoma SH-SY5Y cells. Our present study is the first to report the therapeutic potential of verbenalin for AD; however, further in-depth research in the and models are required to confirm our preliminary findings.
阿尔茨海默病（AD）随着人口老龄化已成为全球主要的健康问题。目前尚无可用的治疗方法可以阻止或逆转 AD 的进展。人羊膜上皮细胞（hAEC）作为干细胞的替代来源，在移植后显示出神经保护和神经修复的潜力。此外，研究表明，用植物源性生物活性化合物对干细胞进行预处理可以增强干细胞的增殖和分化能力，并提高干细胞治疗疾病的能力。马鞭草苷是马鞭草科药用植物中的一种环烯醚萜苷。在本研究中，我们对马鞭草苷处理的 hAEC 进行了微阵列基因表达谱分析，以探索其治疗 AD 的潜力。基因集富集分析显示，马鞭草苷处理显著富集了与 AD 相关的基因集。与溶酶体功能障碍、病理性血管生成、病理性蛋白聚集、昼夜节律、与年龄相关的神经代谢和神经发生相关的基因在马鞭草苷处理的 hAEC 中与对照细胞相比差异表达。此外，马鞭草苷对人神经母细胞瘤 SH-SY5Y 细胞中淀粉样β诱导的神经毒性具有神经保护作用。本研究首次报道了马鞭草苷治疗 AD 的潜力；然而，需要在 和 模型中进行进一步的深入研究来证实我们的初步发现。
