College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China.
Gansu Research Center for Swine Production Engineering and Technology, Lanzhou 730070, China.
Genes (Basel). 2020 Mar 26;11(4):352. doi: 10.3390/genes11040352.
Heme oxygenase 1 () is a stress-inducing enzyme with multiple cardiovascular protective functions, especially in hypoxia stress. However, transcriptional regulation of swine gene remains unclear. In the present study, we first detected tissue expression profiles of gene in adult Hezuo Tibetan pig and analyzed the gene structure. We found that the expression level of gene was highest in the spleen of the Hezuo Tibetan pig, followed by liver, lung, and kidney. A series of 5' deletion promoter plasmids in pGL3-basic vector were used to identify the core promoter region and confirmed that the minimum core promoter region of swine gene was located at -387 bp to -158 bp region. Then we used bioinformatics analysis to predict transcription factors in this region. Combined with site-directed mutagenesis and RNA interference assays, it was demonstrated that the three transcription factors WT1, Sp1 and C/EBPα were important transcription regulators of gene. In summary, our study may lay the groundwork for further functional study of gene.
血红素加氧酶 1（）是一种具有多种心血管保护功能的应激诱导酶，尤其在缺氧应激时。然而，猪基因的转录调控仍不清楚。本研究首先检测了成年合作藏猪中基因的组织表达谱，并分析了基因结构。结果表明，合作藏猪基因在脾脏中的表达水平最高，其次是肝脏、肺和肾脏。我们使用一系列 pGL3-basic 载体中的 5'缺失启动子质粒来鉴定核心启动子区域，并证实了猪基因的最小核心启动子区域位于-387 bp 至-158 bp 区域。然后，我们利用生物信息学分析预测了该区域的转录因子。结合定点突变和 RNA 干扰实验，证明了三个转录因子 WT1、Sp1 和 C/EBPα 是基因的重要转录调节因子。总之，我们的研究可能为进一步研究基因的功能奠定基础。
