• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

利用微囊藻基因拷贝数判断潜在毒性水华，以浅营养化湖泊派尔努湖为例。

Using Microcystin Gene Copies to Determine Potentially-Toxic Blooms, Example from a Shallow Eutrophic Lake Peipsi.

机构信息

Chair of Hydrobiology and Fishery, Institute of Agricultural and Environmental Sciences, Estonian University of Life Sciences, 51006 Tartu, Estonia.

Department F.-A. Forel for Environmental and Aquatic Sciences, University of Geneva, 1205 Geneva, Switzerland.

出版信息

Toxins (Basel). 2020 Mar 26;12(4):211. doi: 10.3390/toxins12040211.

DOI:10.3390/toxins12040211
PMID:32225013
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7232469/
Abstract

Global warming, paired with eutrophication processes, is shifting phytoplankton communities towards the dominance of bloom-forming and potentially toxic cyanobacteria. The ecosystems of shallow lakes are especially vulnerable to these changes. Traditional monitoring via microscopy is not able to quantify the dynamics of toxin-producing cyanobacteria on a proper spatio-temporal scale. Molecular tools are highly sensitive and can be useful as an early warning tool for lake managers. We quantified the potential microcystin (MC) producers in Lake Peipsi using microscopy and quantitative polymerase chain reaction (qPCR) and analysed the relationship between the abundance of the genes, MC concentration, MC variants and toxin quota per gene. We also linked environmental factors to the cyanobacteria community composition. In Lake Peipsi, we found rather moderate MC concentrations, but microcystins and microcystin-producing cyanobacteria were widespread across the lake. Nitrate (NO) was a main driver behind the cyanobacterial community at the beginning of the growing season, while in late summer it was primarily associated with the soluble reactive phosphorus (SRP) concentration. A positive relationship was found between the MC quota per gene and water temperature. The most abundant variant-MC-RR-was associated with MC quota per gene, while other MC variants did not show any significant impact.

摘要

全球变暖与富营养化过程相结合，正促使浮游植物群落向形成水华和潜在有毒蓝藻的优势种转变。浅水湖泊的生态系统尤其容易受到这些变化的影响。传统的显微镜检测无法在适当的时空尺度上量化产毒蓝藻的动态。分子工具具有高度的敏感性，可以作为湖泊管理者的预警工具。我们使用显微镜和定量聚合酶链反应 (qPCR) 来量化派伊兹湖 (Peipsi Lake) 中潜在的微囊藻毒素 (MC) 产生菌，并分析基因丰度、MC 浓度、MC 变体和每个基因的毒素配额之间的关系。我们还将环境因素与蓝藻群落组成联系起来。在派伊兹湖中，我们发现了相当温和的 MC 浓度，但微囊藻和产微囊藻的蓝藻在整个湖中广泛存在。硝酸盐 (NO) 是生长季节初期蓝藻群落的主要驱动因素，而到了夏末，它主要与可溶性反应磷 (SRP) 浓度有关。我们发现每个基因的 MC 配额与水温之间呈正相关关系。最丰富的变体-MC-RR-与每个基因的 MC 配额有关，而其他 MC 变体则没有显示出任何显著的影响。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/adb1/7232469/9e71d6fef1a2/toxins-12-00211-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/adb1/7232469/a3b4b0ad9ec8/toxins-12-00211-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/adb1/7232469/9060fd7f76d6/toxins-12-00211-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/adb1/7232469/7cf0d04bb0cc/toxins-12-00211-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/adb1/7232469/5b4d2e2d8fc7/toxins-12-00211-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/adb1/7232469/c304908c07b0/toxins-12-00211-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/adb1/7232469/694e6b94012c/toxins-12-00211-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/adb1/7232469/9e71d6fef1a2/toxins-12-00211-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/adb1/7232469/a3b4b0ad9ec8/toxins-12-00211-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/adb1/7232469/9060fd7f76d6/toxins-12-00211-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/adb1/7232469/7cf0d04bb0cc/toxins-12-00211-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/adb1/7232469/5b4d2e2d8fc7/toxins-12-00211-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/adb1/7232469/c304908c07b0/toxins-12-00211-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/adb1/7232469/694e6b94012c/toxins-12-00211-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/adb1/7232469/9e71d6fef1a2/toxins-12-00211-g007.jpg

相似文献

1
Using Microcystin Gene Copies to Determine Potentially-Toxic Blooms, Example from a Shallow Eutrophic Lake Peipsi.利用微囊藻基因拷贝数判断潜在毒性水华，以浅营养化湖泊派尔努湖为例。
Toxins (Basel). 2020 Mar 26;12(4):211. doi: 10.3390/toxins12040211.
2
Comparison of cyanobacterial microcystin synthetase (mcy) E gene transcript levels, mcy E gene copies, and biomass as indicators of microcystin risk under laboratory and field conditions.比较蓝藻微囊藻毒素合成酶（mcy）E基因转录水平、mcy E基因拷贝数和生物量，以此作为实验室和野外条件下微囊藻毒素风险的指标。
Microbiologyopen. 2014 Aug;3(4):411-25. doi: 10.1002/mbo3.173. Epub 2014 May 17.
3
Detection of microcystin-producing cyanobacteria in Finnish lakes with genus-specific microcystin synthetase gene E (mcyE) PCR and associations with environmental factors.利用属特异性微囊藻毒素合成酶基因E（mcyE）PCR检测芬兰湖泊中产微囊藻毒素的蓝藻及其与环境因子的关联
Appl Environ Microbiol. 2006 Sep;72(9):6101-10. doi: 10.1128/AEM.01058-06.
4
Seasonal dynamics of toxigenic Microcystis in a large, shallow Lake Peipsi (Estonia) using microcystin mcyE gene abundance.利用微囊藻毒素mcyE基因丰度研究爱沙尼亚大型浅水佩普西湖中产毒微囊藻的季节动态。
Environ Monit Assess. 2024 Jul 18;196(8):747. doi: 10.1007/s10661-024-12909-3.
5
Microcystin mcyA and mcyE Gene Abundances Are Not Appropriate Indicators of Microcystin Concentrations in Lakes.微囊藻毒素mcyA和mcyE基因丰度并非湖泊中微囊藻毒素浓度的合适指标。
PLoS One. 2015 May 6;10(5):e0125353. doi: 10.1371/journal.pone.0125353. eCollection 2015.
6
Environmental influence on cyanobacteria abundance and microcystin toxin production in a shallow temperate lake.环境对浅水温带湖泊中蓝藻丰度和微囊藻毒素产生的影响。
Ecotoxicol Environ Saf. 2015 Apr;114:318-25. doi: 10.1016/j.ecoenv.2014.05.004. Epub 2014 Jul 22.
7
Nitrogen limitation, toxin synthesis potential, and toxicity of cyanobacterial populations in Lake Okeechobee and the St. Lucie River Estuary, Florida, during the 2016 state of emergency event.氮限制、毒素合成潜力以及 2016 年紧急状态期间佛罗里达州奥基乔比湖和圣卢西河河口蓝藻种群的毒性。
PLoS One. 2018 May 23;13(5):e0196278. doi: 10.1371/journal.pone.0196278. eCollection 2018.
8
Is a Central Sediment Sample Sufficient? Exploring Spatial and Temporal Microbial Diversity in a Small Lake.湖心沉积物采样是否足够？探究小湖泊的时空微生物多样性。
Toxins (Basel). 2020 Sep 9;12(9):580. doi: 10.3390/toxins12090580.
9
A Multiplex Analysis of Potentially Toxic Cyanobacteria in Lake Winnipeg during the 2013 Bloom Season.温尼伯湖 2013 年水华季节中潜在有毒蓝藻的多重分析。
Toxins (Basel). 2019 Oct 11;11(10):587. doi: 10.3390/toxins11100587.
10
Estimating microcystin levels at recreational sites in western Lake Erie and Ohio.估算伊利湖西部和俄亥俄州娱乐区的微囊藻毒素水平。
Harmful Algae. 2016 Sep;58:23-34. doi: 10.1016/j.hal.2016.07.003. Epub 2016 Aug 6.

引用本文的文献

1
Cladoceran and Colonial Cyanobacteria: Potentially a Toxic Relationship?枝角类动物与群体蓝藻：潜在的毒性关系？
Toxins (Basel). 2025 Jun 12;17(6):298. doi: 10.3390/toxins17060298.
2
Molecular Quantification of Total and Toxigenic Using Digital-Droplet-Polymerase-Chain-Reaction-Based Multiplex Assay.基于数字液滴聚合酶链反应的多重检测法对总毒素和产毒毒素进行分子定量分析
Toxins (Basel). 2025 May 12;17(5):242. doi: 10.3390/toxins17050242.
3
Crustacean Zooplankton Ingestion of Potentially Toxic : In Situ Estimation Using Gene Gut Content Detection in a Large Temperate Eutrophic Lake.

本文引用的文献

1
Intraspecific variability in response to phosphorus depleted conditions in the cyanobacteria Microcystis aeruginosa and Raphidiopsis raciborskii.在蓝藻门微囊藻和鱼腥藻对缺磷条件的反应中种内变异性。
Harmful Algae. 2019 Jun;86:96-105. doi: 10.1016/j.hal.2019.03.006. Epub 2019 May 31.
2
Spatial analysis of toxic or otherwise bioactive cyanobacterial peptides in Green Bay, Lake Michigan.密歇根湖绿湾有毒或具有其他生物活性的蓝藻肽的空间分析。
J Great Lakes Res. 2018 Oct;44(5):924-933. doi: 10.1016/j.jglr.2018.08.016. Epub 2018 Sep 26.
3
Eutrophication: A new wine in an old bottle?
甲壳类浮游动物对潜在有毒物质的摄食：利用基因肠道内容物检测对大型温带富营养湖泊进行原位估计
Toxins (Basel). 2025 Jan 16;17(1):42. doi: 10.3390/toxins17010042.
4
Gill Junction Injury and Microbial Disorders Induced by Microcystin-Leucine Arginine in Tadpoles.微囊藻氨酸亮氨酸精氨酸引起的蝌蚪吉尔接头损伤和微生物紊乱。
Toxins (Basel). 2022 Jul 13;14(7):479. doi: 10.3390/toxins14070479.
富营养化：旧瓶中的新酒？
Sci Total Environ. 2019 Feb 15;651(Pt 1):1-11. doi: 10.1016/j.scitotenv.2018.09.139. Epub 2018 Sep 13.
4
Cyanobacterial blooms.蓝藻水华。
Nat Rev Microbiol. 2018 Aug;16(8):471-483. doi: 10.1038/s41579-018-0040-1.
5
Temperature Effects Explain Continental Scale Distribution of Cyanobacterial Toxins.温度效应解释了蓝藻毒素的大陆尺度分布。
Toxins (Basel). 2018 Apr 13;10(4):156. doi: 10.3390/toxins10040156.
6
Factors controlling the three-decade long rise in cyanobacteria biomass in a eutrophic shallow lake.控制富营养化浅水湖中蓝藻生物量 30 年长时间增长的因素。
Sci Total Environ. 2018 Apr 15;621:352-359. doi: 10.1016/j.scitotenv.2017.11.250. Epub 2017 Nov 27.
7
Mitigating cyanobacterial harmful algal blooms in aquatic ecosystems impacted by climate change and anthropogenic nutrients.减轻气候变化和人为营养物质影响的水生生态系统中蓝藻有害藻华。
Harmful Algae. 2016 Apr;54:213-222. doi: 10.1016/j.hal.2015.09.009.
8
How rising CO and global warming may stimulate harmful cyanobacterial blooms.随着二氧化碳浓度升高和全球变暖，可能会刺激有害蓝藻水华的形成。
Harmful Algae. 2016 Apr;54:145-159. doi: 10.1016/j.hal.2015.12.006.
9
Can mixtures of cyanotoxins represent a risk to the zooplankton? The case study of Daphnia magna Straus exposed to hepatotoxic and neurotoxic cyanobacterial extracts.蓝藻毒素混合物会对浮游动物构成风险吗？以大型溞暴露于具有肝毒性和神经毒性的蓝藻提取物为例的研究。
Harmful Algae. 2014 Jan;31:143-152. doi: 10.1016/j.hal.2013.11.004. Epub 2013 Dec 1.
10
Trophic state and geographic gradients influence planktonic cyanobacterial diversity and distribution in New Zealand lakes.营养状态和地理梯度影响新西兰湖泊中浮游蓝藻的多样性和分布。
FEMS Microbiol Ecol. 2017 Feb;93(2). doi: 10.1093/femsec/fiw234. Epub 2016 Nov 16.