Walker R J, Lazzaro V A, Duggin G G, Horvath J S, Tiller D J
Department of Renal Medicine, Royal Prince Alfred Hospital, Camperdown, Australia.
Res Commun Chem Pathol Pharmacol. 1988 Dec;62(3):447-60.
Cyclosporin A (CSA) nephrotoxicity was examined in two renal epithelial cell cultures, the LLC-PK1 cell line and the MDCK cell line. Acute changes in cell growth and cellular DNA and protein synthesis were investigated at 2 hours, 20 hours and 5 days. The potential synergistic interaction between streptomycin (a standard additive to most culture media) and CSA was examined. CSA produced significant alterations in cell function as early as 2 hours after exposure and this became more noticeable with increased exposure to CSA. Streptomycin potentiated the toxicity effects on cellular metabolism that was seen with CSA. The use of cell culture models to investigate CSA toxicity must avoid the use of potential agents which may have a synergistic effect on the development of toxicity.
在两种肾上皮细胞培养物（LLC-PK1细胞系和MDCK细胞系）中检测了环孢素A（CSA）的肾毒性。在2小时、20小时和5天时研究了细胞生长以及细胞DNA和蛋白质合成的急性变化。检测了链霉素（大多数培养基的标准添加剂）与CSA之间潜在的协同相互作用。CSA在暴露后2小时就对细胞功能产生了显著改变，并且随着对CSA暴露时间的增加，这种改变变得更加明显。链霉素增强了CSA对细胞代谢的毒性作用。利用细胞培养模型研究CSA毒性时必须避免使用可能对毒性发展有协同作用的潜在试剂。
