Coles G C, Tritschler J P, Giordano D J, Laste N J, Schmidt A L
Department of Zoology, University of Massachusetts, Amherst 01003.
Res Vet Sci. 1988 Jul;45(1):50-3.
The growth, using freshly cultured Escherichia coli with ampicillin or heat-treated lyophilised E coli as a food source, of the larvae of the mouse nematode Nematospiroides dubius and the infectivity of resulting third stage larvae were determined. Concentrations of E coli between 0.5 and 1 mg dry weight ml-1 permitted optimal larval development for both N dubius and Trichostrongylus colubriformis. Development of larvae of susceptible and cambendazole-resistant strains of Haemonchus contortus in thiabendazole solutions showed clear differences between the strains and the larval development test was more sensitive than the egg hatch test. The test also detected a levamisole resistant strain of H contortus, although the degree of resistance could not be adequately measured. It is concluded that the test can be run with any anthelmintic to which resistance is suspected.
以新鲜培养的含氨苄青霉素的大肠杆菌或经热处理的冻干大肠杆菌作为食物来源，测定了小鼠线虫杜氏旋毛虫幼虫的生长情况以及由此产生的第三期幼虫的感染性。大肠杆菌浓度在0.5至1毫克干重/毫升之间时，有利于杜氏旋毛虫和蛇形毛圆线虫幼虫的最佳发育。在噻苯达唑溶液中，捻转血矛线虫敏感株和抗坎苯达唑株幼虫的发育表现出明显的菌株差异，且幼虫发育试验比虫卵孵化试验更敏感。该试验还检测到一株捻转血矛线虫左旋咪唑抗性株，尽管其抗性程度无法准确测定。得出的结论是，该试验可用于任何怀疑有抗药性的驱虫药。
