Opt Express. 2020 Mar 30;28(7):9269-9279. doi: 10.1364/OE.389043.
This work proposes a novel fluorescence Scheimpflug LiDAR (SLiDAR) technique based on the Scheimpflug principle for three-dimension (3D) plant profile measurements. A 405 nm laser diode was employed as the excitation light source to generate a light sheet. Both the elastic and inelastic/fluorescence signals from a target object (e.g., plants) can be simultaneously measured by the fluorescence SLiDAR system employing a color image sensor with blue, green and red detection channels. The 3D profile can be obtained from the elastic signal recorded by blue pixels through elevation scanning measurements, while the fluorescence intensity of the target object is mainly acquired by red and green pixels. The normalized fluorescence intensity of the red channel, related to the chlorophyll distribution of the plant, can be utilized for the classification of leaves, branches and trunks. The promising results demonstrated in this work have shown a great potential of employing the fluorescence SLiDAR technique for 3D fluorescence profiling of plants in agriculture and forestry applications.
本文提出了一种基于 Scheimpflug 原理的新型荧光 Scheimpflug 激光雷达 (SLiDAR) 技术，用于三维 (3D) 植物轮廓测量。采用 405nm 激光二极管作为激发光源产生光片。荧光 SLiDAR 系统采用具有蓝色、绿色和红色探测通道的彩色图像传感器，可同时测量目标物体（如植物）的弹性和非弹性/荧光信号。通过对蓝色像素记录的弹性信号进行高程扫描测量，可获得 3D 轮廓，而目标物体的荧光强度主要由红色和绿色像素获得。与植物叶绿素分布相关的红色通道归一化荧光强度可用于叶片、树枝和树干的分类。本文的研究结果表明，荧光 SLiDAR 技术在农业和林业应用中对植物的 3D 荧光轮廓进行测量具有很大的潜力。
