建立二倍体和四倍体金银花的色谱指纹图谱用于质量分析。
Development of chromatographic fingerprint for quality analysis of diploid and tetraploid Lonicera japonica.
机构信息
Institute of Tropical Crop Genetic Resources, Chinese Academy of Tropical Agriculture Sciences, Danzhou 571737, China.
School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China.
出版信息
J Tradit Chin Med. 2020 Feb;40(1):73-82.
OBJECTIVE
To develop the chromatographic fingerprint of Lonicera japonica (L. japonica) and evaluate the effects of polyploidy on the quality of L. japonica.
METHODS
High-performance liquid chromatography (HPLC) methods used to establish the chromatographic fingerprint were developed. The quality of 11 batches of diploid L. japonica and 13 batches of tetraploid L. japonica collected from different regions across China were analyzed. The contents of five active compounds, consisting of chlorogenic acid, rutin, galuteolin, isochlorogenic acid A and quercetin, were further detected in L. japonica.
RESULTS
The chromatographic fingerprint established by the optimized HPLC method was verified for qualitative analysis of L. japonica. Quantitative analysis showed that the contents of chlorogenic acid, isochlorogenic acid A, and quercetin in tetraploid plants were higher than those in diploid plants, whereas rutin and galuteolin contents in tetraploid plants were lower than those in diploid plants.
CONCLUSION
The developed HPLC method is suitable for qualitative analysis of L. japonica. Polyploidy was indicated to influence the chemical properties of L. japonica. Tetraploid L. japonica shows potential for utilization as a medicinal plant with different active components.
目的
建立金银花的色谱指纹图谱，并评估多倍体对金银花质量的影响。
方法
采用高效液相色谱法（HPLC）建立色谱指纹图谱。分析了来自中国不同地区的 11 批二倍体金银花和 13 批四倍体金银花的质量。进一步检测了金银花中 5 种活性成分（绿原酸、芦丁、木犀草素、异绿原酸 A 和槲皮素）的含量。
结果
优化后的 HPLC 方法建立的色谱指纹图谱可用于金银花的定性分析。定量分析表明，四倍体植物中绿原酸、异绿原酸 A 和槲皮素的含量高于二倍体植物，而四倍体植物中芦丁和木犀草素的含量低于二倍体植物。
结论
所建立的 HPLC 方法适用于金银花的定性分析。多倍体影响了金银花的化学性质。四倍体金银花具有作为药用植物的潜力，具有不同的活性成分。
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