miR-92a-3p、氧化应激和 p38MAPK/NF-κB 通路在大鼠中心静脉导管相关性血栓形成中的作用。

Role of miR-92a-3p, oxidative stress, and p38MAPK/NF-κB pathway in rats with central venous catheter related thrombosis.

机构信息

Department of Cardiothoracic surgery, The First Affiliated Hospital of Guangxi Medical University, No. 6 Shuangyong Road, Nanning, 530021, China.

出版信息

BMC Cardiovasc Disord. 2020 Mar 30;20(1):150. doi: 10.1186/s12872-020-01436-x.

BACKGROUND

miR-92a-3p and oxidative stress are reportedly associated with venous thrombosis. However, the role of miR-92a-3p and oxidative stress in catheter-related thrombosis (CRT) remains ambiguous. Herein, we studied the roles of miR-92a-3p, oxidative stress, and p38-mitogen-activated protein kinase/nuclear factor kappa-B (MAPK/NF-κB) pathway in CRT.

METHODS

Forty-five male rats were randomly and equally divided into control, sham operation, and CRT groups. The rats were sacrificed after 10 days. Reactive oxygen species (ROS), superoxide dismutase (SOD), and malondialdehyde (MDA) levels in the serum were determined by enzyme-linked immunosorbent assay (ELISA). The expression levels of miR-92a-3p, heme oxygenase-1 (HO-1), NF-κB p65, and p38 MAPK in the venous tissues were detected with quantitative polymerase chain reaction (qPCR) and Western blot.

RESULTS

Thrombosis was observed only in the CRT group. Compared with the levels in the control and sham operation groups, ROS and MDA significantly increased in the CRT group, but SOD significantly decreased. qPCR and Western blot results showed that miR-92a-3p, HO-1, p38 MAPK, and NF-κB p65 expression was significantly upregulated in the venous tissues of the CRT group. Moreover, miR-92a-3p was positively correlated with HO-1, which was positively correlated with p38 MAPK and NF-κB p65.

CONCLUSION

miR-92a-3p was correlated with oxidative stress in CRT. miR-92a-3p and oxidative stress contributed to endothelial dysfunction and simultaneously was associated with CRT.

背景

miR-92a-3p 和氧化应激据称与静脉血栓形成有关。然而，miR-92a-3p 和氧化应激在导管相关性血栓形成（CRT）中的作用仍不清楚。在此，我们研究了 miR-92a-3p、氧化应激和 p38-丝裂原活化蛋白激酶/核因子 kappa-B（MAPK/NF-κB）通路在 CRT 中的作用。

方法

45 只雄性大鼠随机均分为对照组、假手术组和 CRT 组。10 天后处死大鼠。采用酶联免疫吸附试验（ELISA）检测血清中活性氧（ROS）、超氧化物歧化酶（SOD）和丙二醛（MDA）的水平。采用实时定量聚合酶链反应（qPCR）和 Western blot 检测静脉组织中 miR-92a-3p、血红素加氧酶-1（HO-1）、NF-κB p65 和 p38 MAPK 的表达水平。

结果

仅在 CRT 组观察到血栓形成。与对照组和假手术组相比，CRT 组 ROS 和 MDA 显著增加，而 SOD 显著降低。qPCR 和 Western blot 结果显示，CRT 组静脉组织中 miR-92a-3p、HO-1、p38 MAPK 和 NF-κB p65 的表达明显上调。此外，miR-92a-3p 与 HO-1 呈正相关，HO-1 与 p38 MAPK 和 NF-κB p65 呈正相关。