School of Materials Science and Engineering, South China University of Technology, Guangzhou 510640, China; Department of Bone and Joint Surgery, Institute of Orthopedic Diseases, The First Affiliated Hospital, Jinan University, Guangzhou 510630, China; National Engineering Research Center for Tissue Restoration and Reconstruction, Guangzhou 510006, China.
College of Chemistry and Materials, Jinan University, Guangzhou 510632, China.
Mater Sci Eng C Mater Biol Appl. 2020 Apr;109:110481. doi: 10.1016/j.msec.2019.110481. Epub 2019 Nov 22.
Lack of osteogenic capacity limits the bone repair effect of calcium phosphate cement (CPC). In present work, bivalent manganese ion (Mn) doped β-tricalcium phosphate (Mn-TCP) was incorporated into CPC to enhance its osteogenic ability. The incorporation of Mn-TCP promoted the hydration reaction of CPC. The presence of Mn made the hydration products finer. When adding 10 wt% Mn-TCP in CPC (Mn-CPC-1), the setting time of CPC was shortened, whereas the strength and injectability were not changed. Mouse Bone marrow mesenchymal stem cells (mBMSCs) on Mn-CPC-1 and CPC with 20 wt% Mn-TCP (Mn-CPC-2) presented better adhesion and spreading behaviors. Besides, Mn-CPC-1 promoted the gene levels of ALP, Col-I and OC while Mn-CPC-2 promoted the gene levels of Runx2 and OC. Cellular behaviors were related to two points: one was the increase of adsorption capacity of proteins (e.g. BSA) after changing the surface properties of bone cements; and the other was the biological role of Mn released from CPC in osteogenesis. All the results indicated that CPC incorporated with 10 wt% Mn-TCP has good osteogenesis and proper physicochemical properties, which will be a prospective biomaterial applying in the area of bone regeneration.
成骨能力的缺乏限制了磷酸钙水泥（CPC）的骨修复效果。在目前的工作中，二价锰离子（Mn）掺杂β-磷酸三钙（Mn-TCP）被掺入 CPC 中以增强其成骨能力。Mn-TCP 的掺入促进了 CPC 的水合反应。Mn 的存在使水合产物更加细化。当 CPC 中添加 10wt% Mn-TCP（Mn-CPC-1）时，CPC 的凝固时间缩短，而强度和可注射性没有改变。Mn-CPC-1 和添加 20wt% Mn-TCP 的 CPC（Mn-CPC-2）上的小鼠骨髓间充质干细胞（mBMSCs）表现出更好的黏附和铺展行为。此外，Mn-CPC-1 促进了 ALP、Col-I 和 OC 的基因水平，而 Mn-CPC-2 则促进了 Runx2 和 OC 的基因水平。细胞行为与两点有关：一是改变骨水泥表面性能后蛋白质（如 BSA）吸附能力的增加；另一个是 CPC 中释放的 Mn 在成骨中的生物学作用。所有结果表明，掺入 10wt% Mn-TCP 的 CPC 具有良好的成骨能力和适当的理化性能，将成为应用于骨再生领域的有前景的生物材料。
