Department of Applied Chemistry, S.V. National Institute of Technology, Surat 395007, Gujarat, India.
Department of Applied Chemistry, S.V. National Institute of Technology, Surat 395007, Gujarat, India.
Mater Sci Eng C Mater Biol Appl. 2020 Apr;109:110645. doi: 10.1016/j.msec.2020.110645. Epub 2020 Jan 7.
Different blood groups of ABO system have specific antigen which bestows them with different biochemical properties and hence they can show different hemolytic activity. In this report, hemolytic activity of thiol-functionalized FeO-Au nanoparticles were studied in presence and absence of doxorubicin and the effect of various thiol coatings were correlated towards their hemolysis tendency. The nanoparticles were functionalized with four different amino thiols, cysteamine (CEA), cystamine (CA), cysteine (Cys) and cystine (Cyt) to form FeO-Au CEA, FeO-Au CA, FeO-Au Cys and FeO-Au Cyt nanoparticles which were loaded with anticancer drug, doxorubicin. The functionalization was characterized using ATR-FTIR, HR-TEM, XPS and other spectroscopic methods. Maximum drug encapsulation efficiency of 83% was observed with FeO-Au CA nanoparticles. In-vitro experiments were performed on HeLa cells to check the cellular uptake and cytotoxicity using MTT assay. Hemolytic activity was then analyzed with all the blood groups (positive and negative). The amino acid functionalized, FeO-Au Cys and FeO-Au Cyt nanoparticles, shows lesser hemolysis compared to amino thiol functionalized FeO-Au CEA, and FeO-Au CA nanoparticles. In positive blood groups, the FeO-Au CA nanoparticles shows the highest rate of hemolysis followed by FeO-Au CEA, while the lowest hemolysis rate was observed for FeO-Au Cyt nanoparticles. For negative blood groups, the thiol coated nanoparticles show more abrupt hemolysis rate depending upon the type of antigen.
不同的 ABO 血型系统具有特定的抗原，赋予它们不同的生化特性，因此它们可以表现出不同的溶血活性。在本报告中，研究了巯基功能化的 FeO-Au 纳米粒子在阿霉素存在和不存在的情况下的溶血活性，并将各种巯基涂层的效果与它们的溶血倾向相关联。纳米粒子分别用四种不同的氨基硫醇，半胱胺（CEA）、胱胺（CA）、半胱氨酸（Cys）和胱氨酸（Cyt）进行功能化，形成 FeO-Au CEA、FeO-Au CA、FeO-Au Cys 和 FeO-Au Cyt 纳米粒子，这些纳米粒子负载有抗癌药物阿霉素。通过 ATR-FTIR、HR-TEM、XPS 和其他光谱方法对功能化进行了表征。用 FeO-Au CA 纳米粒子观察到最大的药物包封效率为 83%。在 HeLa 细胞上进行了体外实验，通过 MTT 测定法检查细胞摄取和细胞毒性。然后用所有血型（阳性和阴性）分析了溶血活性。与氨基酸功能化的 FeO-Au CEA 和 FeO-Au CA 纳米粒子相比，氨基酸功能化的 FeO-Au Cys 和 FeO-Au Cyt 纳米粒子的溶血活性较低。在阳性血型中，FeO-Au CA 纳米粒子的溶血率最高，其次是 FeO-Au CEA，而 FeO-Au Cyt 纳米粒子的溶血率最低。对于阴性血型，根据抗原的类型，巯基涂层纳米粒子显示出更高的溶血率。
