Department of Chemical Engineering , University of Science and Technology of Mazandaran , Behshahr 4851878195 , Iran.
Department of Chemistry, Faculty of Science , University of Qom , Qom 4661137161 , Iran.
Langmuir. 2018 Nov 13;34(45):13735-13744. doi: 10.1021/acs.langmuir.8b02390. Epub 2018 Nov 1.
Herein, we developed a magnetic drug delivery system based on magnetic FeO nanoparticles with double shells of modified salep polysaccharide for the delivery of doxorubicin (Dox). The drug-loaded nanocarrier was synthesized in an easy way, and large amounts of drug molecules were loaded into the nanocarrier. The drug-loaded nanocarrier showed excellent pH responsibility in vitro, and large amounts of Dox were released at lower pH (60% release), whereas the nanocarrier was stable at neutral pH. The hemolysis assay results showed that the nanocarrier has negligible hemolytic effects on human red blood cells and showed good biocompatibility. Moreover, the result of coagulation assays showed that the nanocarrier was not active in any coagulation pathways. Cytotoxicity assays of nanocarrier and drug-loaded nanocarrier toward HeLa cells demonstrated that the nanocarrier has negligible toxicity, whereas the drug-loaded nanocarrier kills more than 90% of cells during 48 h. The flow cytometry analysis also showed that the uptake of drug-loaded nanocarrier into the cancerous cells is time-dependent and higher concentrations of drug internalized into the cells at longer incubation time. On the basis of the results, we suggest that the present nanocarrier can be applicable for in vivo drug delivery as an easy-made and cheap nanocarrier.
在此，我们开发了一种基于具有改性薤白多糖双层壳的磁性 FeO 纳米粒子的磁药物递送系统，用于递送阿霉素（Dox）。载药纳米载体通过简单的方法合成，并将大量药物分子载入纳米载体中。载药纳米载体在体外表现出优异的 pH 响应性，在较低 pH（60%释放）下大量释放 Dox，而纳米载体在中性 pH 下稳定。溶血试验结果表明，纳米载体对人红细胞几乎没有溶血作用，表现出良好的生物相容性。此外，凝血试验结果表明纳米载体在任何凝血途径中均不活跃。纳米载体和载药纳米载体对 HeLa 细胞的细胞毒性试验表明，纳米载体几乎没有毒性，而载药纳米载体在 48 小时内杀死超过 90%的细胞。流式细胞术分析还表明，载药纳米载体进入癌细胞的摄取是时间依赖性的，并且在更长的孵育时间内，细胞内内化的药物浓度更高。基于这些结果，我们认为该纳米载体可作为一种易于制备且廉价的纳米载体，适用于体内药物递送。
