School of Public Health, Nanjing Medical University, 211166 Nanjing, China.
National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China; College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China.
Mater Sci Eng C Mater Biol Appl. 2020 Apr;109:110627. doi: 10.1016/j.msec.2020.110627. Epub 2020 Jan 7.
In response to extreme environmental conditions, Bactrian camels with largest population in China have evolved with the unique and extraordinary stress-tolerant mechanism in the bodies, in which the most abundantly secreted serum albumins contribute to an important role in diverse physiological activities such as maintaining osmotic pressure and transporting endogenous/exogenous molecules. In this study, we have for the first time purified Chinese Bactrian camel serum albumins (CSA) aimed at exploring their biomedical application. The mass spectrometric as well as structural analysis of CSA have revealed the sequence consensus and alpha-helix abundant structures among its heterologous proteins. Using desolvation methods, CSA-based nanoparticles have been prepared to encapsulate two substrate molecules including Doxorubicin (Dox) and hemin, which confers the versatility of nanocomposite. As drug delivery matrix, the Dox-loaded CSA nanoparticles displayed sustained release behaviors of DOX with the decreased cytotoxicity detected by both CCK-8 assay and real-time cell analysis. The CSA-hemin nanoparticles exhibited superior catalytic activities in the oxidation of Orange II comparable with horse radish peroxidase following a ping-pong mechanism. Furthermore, the constructed CSA-hemin nanoparticles were applied for the spectroscopic detection of HO resulting in a wide linear calibration curve ranging from 5 to 400 μM with a detection limit of 3.32 μM.
针对极端环境条件，中国数量最多的双峰驼已经进化出独特而非凡的应激耐受机制，其中最丰富分泌的血清白蛋白在维持渗透压和运输内源性/外源性分子等多种生理活动中发挥重要作用。在这项研究中，我们首次纯化了中国双峰驼血清白蛋白（CSA），旨在探索其在生物医学中的应用。CSA 的质谱和结构分析揭示了其异源蛋白的序列一致性和富含α-螺旋的结构。使用去溶剂化方法，制备了基于 CSA 的纳米颗粒来包封两种基质分子，包括阿霉素（Dox）和血红素，赋予了纳米复合材料的多功能性。作为药物输送基质，负载 Dox 的 CSA 纳米颗粒表现出 DOX 的持续释放行为，通过 CCK-8 测定法和实时细胞分析检测到降低的细胞毒性。CSA-血红素纳米颗粒在 Orange II 的氧化中表现出与辣根过氧化物酶相当的优异催化活性，遵循乒乓机制。此外，构建的 CSA-血红素纳米颗粒用于 HO 的光谱检测，导致在 5 至 400 μM 的宽线性校准曲线，检测限为 3.32 μM。
