Research Institute of Applied Chemistry, Shanxi University, Taiyuan 030006, China.
Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Molecular Science, Shanxi University, Taiyuan 030006, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2020 Jun 15;234:118253. doi: 10.1016/j.saa.2020.118253. Epub 2020 Mar 12.
Sulfur dioxide (SO) maintains a certain steady state balance in the body, high concentration SO will be harmful to human health. Seeking a suitable detection method to monitor sulfur dioxide in real time becomes an urgent requirement owing to the transient nature of sulfur dioxide in organisms. Here, a novel NIR ratiometric fluorescent probe for detection of SO was developed based on a conjugation of coumarin and indol salt with excellent water solubility. The probe Mito-CI displayed highly sensitive (69 nM), fast response time (30 s), large Stokes shift (174 nm) and the NIR fluorescence emission wavelength (655 nm). In the reversibility process of the SO-probe Mito-CI system induced by HCHO in vitro was also detected. Besides, cell imaging showed that Mito-CI possesses mitochondria-targeted ability. Particularly, Mito-CI was proved to reversibly detect SO/HCHO in living cells.
二氧化硫（SO）在体内保持着一定的稳态平衡，高浓度的 SO 会对人体健康造成危害。由于二氧化硫在生物体内的瞬态性质，寻找一种合适的检测方法来实时监测二氧化硫成为当务之急。在这里，我们基于香豆素和吲哚盐的共轭物开发了一种新型的近红外比率荧光探针来检测 SO，该探针具有出色的水溶性。探针 Mito-CI 表现出高灵敏度（69 nM）、快速响应时间（30 s）、大斯托克斯位移（174nm）和近红外荧光发射波长（655nm）。此外，还检测了体外 HCHO 诱导的 SO-探针 Mito-CI 体系的可逆性过程。此外，细胞成像表明 Mito-CI 具有线粒体靶向能力。特别是，Mito-CI 被证明可以在活细胞中可逆地检测 SO/HCHO。
