Institute of Fluorescent Probes for Biological Imaging, School of Chemistry and Chemical Engineering, School of Materials Science and Engineering , University of Jinan , Jinan , Shandong 250022 , People's Republic of China.
Anal Chem. 2019 Aug 20;91(16):10723-10730. doi: 10.1021/acs.analchem.9b02119. Epub 2019 Aug 1.
Living systems contain a diverse array of molecules and ions undergoing dynamic changes by a web of interacting chemical reactions. Sulfur dioxide (SO) and formaldehyde (FA) can be generated endogenously in living organisms to maintain their homeostasis, but aberrant production of these species is implicated with some critical diseases. The dynamic interaction between SO and FA on the overall health and disease of living organisms remains challenging to elucidate owing to a dearth of methods for monitoring dynamic fluctuation of these transient species. Herein, we present the rational design, synthesis, and photophysical property studies of the probe (), the first reversible fluorescent probe for investigating the dynamics changes of SO and FA. Importantly, the highly desirable attributes of the robust probe (such as ultrafast response to SO in less than 5 s, swift restoration by FA in less than 1 min) make it possible to reversibly monitor the dynamic fluctuation of endogenous SO and FA in real-time in living cells for the first time. Moreover, we demonstrate the utility of this unique probe to detect the fluctuations of SO and FA in living zebrafish and murine species. This work provides a powerful chemical tool for monitoring the dynamic interaction of endogenous SO and FA, which will pave an avenue for interrogating the intersecting correlation between SO and FA in health and disease states.
生命系统中含有多种分子和离子，它们通过相互作用的化学反应网络发生动态变化。二氧化硫 (SO) 和甲醛 (FA) 可以在活生物体中内源性产生，以维持其体内平衡，但这些物质的异常产生与一些严重疾病有关。由于缺乏监测这些瞬态物质动态波动的方法，因此阐明 SO 和 FA 对生物体整体健康和疾病的动态相互作用仍然具有挑战性。在此，我们提出了探针 () 的合理设计、合成和光物理性质研究，这是第一个用于研究 SO 和 FA 动力学变化的可逆荧光探针。重要的是，该强稳健探针 () 具有超快的响应（不到 5 秒即可响应 SO）和快速的恢复（不到 1 分钟即可被 FA 恢复），这使得首次能够实时在活细胞中可逆监测内源性 SO 和 FA 的动态波动成为可能。此外，我们证明了该独特探针在检测活体斑马鱼和鼠类物种中 SO 和 FA 波动方面的实用性。这项工作为监测内源性 SO 和 FA 的动态相互作用提供了一种强大的化学工具，这将为研究健康和疾病状态下 SO 和 FA 之间的交叉相关性开辟一条道路。
