Qilu University of Technology (Shandong Academy of Sciences), Shandong Analysis and Test Center, Jinan 250014, PR China; Key Laboratory of Colloid and Interface Chemistry, Shandong University, Ministry of Education, Jinan 250100, PR China.
Qilu University of Technology (Shandong Academy of Sciences), Shandong Analysis and Test Center, Jinan 250014, PR China.
Food Chem. 2020 Aug 1;320:126683. doi: 10.1016/j.foodchem.2020.126683. Epub 2020 Mar 23.
Here, we demonstrate use of acetylcholinesterase (AChE)-responsive polyoxometalate (POM)/surfactant supramolecular spheres to build a liquid crystal (LC)-based sensing platform for detection of organophosphorus pesticides. The self-assembled spheres are composed of hybrid materials of a POM, sodium dodecatungstophosphate (PW), and a surfactant, myristoylcholine (Myr). It displays dark appearance when the aqueous solution is in contact with LCs supported on the octadecyltrichlorosilane-treated glass deposited with the supramolecular spheres, suggesting perpendicular orientation of LCs at the aqueous/LC interface. In contrast, LCs show bright appearance when the surface-deposited supramolecular spheres are enzymatically hydrolyzed by AChE, corresponding to planar orientation of LCs at the aqueous/LC interface. Detection of organophosphates are successfully achieved as they are potent inhibitors of AChE. The detection limit of the sensing platform reached 0.9 ng/mL for dimethoate. This method can avoid disturbance of external interference with excellent specificity and sensitivity, which makes it very promise in detection of organophosphorus pesticides.
在这里，我们展示了使用乙酰胆碱酯酶（AChE）响应的多金属氧酸盐（POM）/表面活性剂超分子球来构建基于液晶（LC）的传感平台，用于检测有机磷农药。自组装的球体由混合材料组成，包括多金属氧酸盐、十二钨酸钠（PW）和表面活性剂、肉豆蔻酰胆碱（Myr）。当水溶液与在十八烷基三氯硅烷处理的玻璃上沉积的超分子球上支撑的 LC 接触时，溶液呈现暗外观，表明 LC 在水/LC 界面处呈垂直取向。相比之下，当表面沉积的超分子球被 AChE 酶解时，LC 呈现亮外观，对应于 LC 在水/LC 界面处的平面取向。成功地实现了对有机磷的检测，因为它们是 AChE 的有效抑制剂。该传感平台的检测限达到 0.9ng/mL 对乐果。该方法可以避免外部干扰的干扰，具有出色的特异性和灵敏度，因此在检测有机磷农药方面非常有前景。
