Key Laboratory of Colloid and Interface Chemistry, Shandong University, Ministry of Education, Jinan 250100, PR China.
Department of Chemistry, University of Houston, Houston, TX 77204, United States.
Biosens Bioelectron. 2015 Oct 15;72:25-30. doi: 10.1016/j.bios.2015.05.001. Epub 2015 May 2.
In this paper, construction of the liquid crystal (LC)-based sensing platform for simple and sensitive detection of acetylcholinesterase (AChE) and its inhibitor using a cationic surfactant-decorated LC interface was demonstrated. A change of the optical images of LCs from bright to dark appearance was observed when the cationic surfactant, myristoylcholine chloride (Myr), was transferred onto the aqueous/LC interface, due to the formation of a stable surfactant monolayer at the interface. A dark-to-bright change of the optical appearance was then observed when AChE was transferred onto the Myr-decorated LC interface. The sensitivity of this new type of LC-based sensor is 3 orders of magnitude higher in the serum albumin solution than that only in the buffer solution. Noteworthy is that the AChE LC sensor shows a very high sensitivity for the detection of the enzyme inhibitor, which is around 1 fM. The constructed low-cost LC-based sensor is quite simple and convenient, showing high promise for label-free detection of AChE and its inhibitors.
本文构建了基于液晶（LC）的传感平台，用于使用阳离子表面活性剂修饰的 LC 界面简单灵敏地检测乙酰胆碱酯酶（AChE）及其抑制剂。当阳离子表面活性剂十四酰胆碱氯化物（Myr）转移到水/LC 界面时，观察到 LC 的光学图像从亮到暗的变化，这是由于界面上形成了稳定的表面活性剂单层。当 AChE 转移到 Myr 修饰的 LC 界面上时，光学外观会从暗变亮。与仅在缓冲溶液中相比，这种新型基于 LC 的传感器在血清白蛋白溶液中的灵敏度高出 3 个数量级。值得注意的是，AChE LC 传感器对酶抑制剂的检测具有非常高的灵敏度，约为 1 fM。所构建的低成本基于 LC 的传感器非常简单方便，在 AChE 及其抑制剂的无标记检测方面具有很高的应用前景。
