Institute for Plant Genetics, Heinrich-Heine University Düsseldorf, Düsseldorf 40225, Germany.
Max-Planck-Institute for Plant Breeding Research, Cologne 50829, Germany.
Plant Physiol. 2020 Jun;183(2):765-779. doi: 10.1104/pp.19.01411. Epub 2020 Mar 30.
The circadian clock is a complex transcriptional network that regulates gene expression in anticipation of the day/night cycle and controls agronomic traits in plants. However, in crops, how the internal clock and day/night cues affect the transcriptome remains poorly understood. We analyzed the and circadian leaf transcriptomes in the barley () cultivar 'Bowman' and derived introgression lines harboring mutations in (), (), and (). The and mutants exhibited abolished circadian transcriptome oscillations under constant conditions, whereas maintained oscillations of ≈30% of the circadian transcriptome. However, day/night cues fully restored transcript oscillations in all three mutants and thus compensated for a disrupted oscillator in the arrhythmic barley clock mutants and Nevertheless, , but not , affected the phase of the oscillating transcriptome and thus the integration of external cues into the clock. Using dynamical modeling, we predicted a structure of the barley circadian oscillator and interactions of its individual components with day/night cues. Our findings provide a valuable resource for exploring the function and output targets of the circadian clock and for further investigations into the and circadian control of the barley transcriptome.
生物钟是一个复杂的转录网络，它可以预测昼夜节律，并控制植物的农艺性状。然而，在作物中，内部时钟和昼夜线索如何影响转录组仍知之甚少。我们分析了大麦（Hordeum vulgare）品种“Bowman”的 和昼夜叶片转录组，并衍生出携带突变的导入系 （）、 （）和 （）。 和 突变体在持续光照条件下表现出生物钟转录组振荡的消除，而 则维持约 30%的生物钟转录组的振荡。然而，昼夜线索完全恢复了所有三个突变体的转录振荡，从而补偿了节律紊乱的大麦生物钟突变体 和 中破坏的振荡器。尽管如此， 而不是 ，影响了振荡转录组的相位，从而将外部线索整合到时钟中。通过动态建模，我们预测了大麦生物钟振荡器的结构以及其各个组成部分与昼夜线索的相互作用。我们的研究结果为探索生物钟的功能和输出靶点以及进一步研究 昼夜节律对大麦转录组的控制提供了有价值的资源。
