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基于细胞色素氧化酶亚基I线粒体DNA条形码和形态学对蜘蛛属Ixchela Huber(蜘蛛目:幽灵蛛科)进行物种界定,并描述来自墨西哥的两个新物种。

COI mtDNA barcoding and morphology for species delimitation in the spider genus Ixchela Huber (Araneae: Pholcidae), with the description of two new species from Mexico.

作者信息

Valdez-Mondragón Alejandro

机构信息

CONACYT Research Fellow. Laboratory of Arachnology, Laboratorio Regional de Biodiversidad y Cultivo de Tejidos Vegetales (LBCTV), Instituto de Biología, Universidad Nacional Autónoma de México (UNAM), sede Tlaxcala, Ex-Fábrica San Manuel, San Miguel Contla, 90640 Santa Cruz Tlaxcala, Tlaxcala, Mexico. Colección Nacional de Arácnidos (CNAN), Departamento de Zoología, Instituto de Biología, Universidad Nacional Autónoma de México (UNAM), Ciudad Universitaria, Apartado Postal 04510, Coyoacán, Mexico City, Mexico..

出版信息

Zootaxa. 2020 Mar 2;4747(1):zootaxa.4747.1.2. doi: 10.11646/zootaxa.4747.1.2.

DOI:10.11646/zootaxa.4747.1.2
PMID:32230118
Abstract

The spider genus Ixchela Huber 2000, is comprised of 22 species distributed from north-eastern Mexico to Central America, including the two new species described herein from Mexico: Ixchela panchovillai sp. nov. and Ixchela zapatai sp. nov., both from the state of Oaxaca and described for both sexes. DNA barcoding utilizing mitochondrial cytochrome c oxidase subunit 1 (COI) and morphology were used for species delimitation. Molecular analyses and species delimitation included four methods: 1) corrected p-distances under neighbor-joining (NJ), 2) general mixed yule coalescent model (GMYC), 3) automatic barcode gap discovery (ABGD), and 4) Poisson tree processes (bPTP). All molecular methods and morphology were consistent in delimiting and recognizing the two new species describing herein. The average inter-specific genetic distance (p-distance) within the genus Ixchela is 12%. Ixchela panchovillai sp. nov. is closely related in the NJ analysis with I. placida, with an average p-distance of 7.9%, whereas I. zapatai sp. nov. is closely related to I. taxco, with an average p-distance of 8.4% between both species. Additionally, identification keys for males and females of the genus Ixchela are presented.

摘要

蜘蛛属伊克切拉(Ixchela)由胡贝尔(Huber)于2000年建立,包含22个物种,分布于墨西哥东北部至中美洲,其中包括本文描述的来自墨西哥的两个新物种:潘乔·比利亚伊克切拉蜘蛛(Ixchela panchovillai sp. nov.)和萨帕塔伊克切拉蜘蛛(Ixchela zapatai sp. nov.),均来自瓦哈卡州,且对两性都进行了描述。利用线粒体细胞色素c氧化酶亚基1(COI)进行DNA条形码分析以及形态学分析用于物种界定。分子分析和物种界定包括四种方法:1)邻接法(NJ)下的校正p距离,2)广义混合尤尔合并模型(GMYC),3)自动条形码间隙发现(ABGD),以及4)泊松树过程(bPTP)。所有分子方法和形态学分析在界定和识别本文描述的两个新物种方面都是一致的。伊克切拉属内种间平均遗传距离(p距离)为12%。在邻接分析中,潘乔·比利亚伊克切拉蜘蛛与平静伊克切拉蜘蛛(I. placida)密切相关,平均p距离为7.9%,而萨帕塔伊克切拉蜘蛛与塔斯科伊克切拉蜘蛛(I. taxco)密切相关。两个物种之间的平均p距离为8.4%。此外,还给出了伊克切拉属雄性和雌性的鉴定检索表。

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