Mettrick Karla, Hassan Karl, Lamont Iain, Reid David
School of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW 2308, Australia.
Department of Biochemistry, University of Otago, Dunedin 9016, New Zealand.
Antibiotics (Basel). 2020 Mar 27;9(4):144. doi: 10.3390/antibiotics9040144.
Targeting the iron requirement of may be an effective adjunctive for conventional antibiotic treatment against biofilm-dwelling . We, therefore, assessed the anti-biofilm activity of N,N'-bis (2-hydroxybenzyl) ethylenediamine-N,N'-diacetic acid (HBED), which is a synthetic hexadentate iron chelator. The effect of HBED was studied using short-term (microtitre plate) and longer-term (flow-cell) biofilm models, under aerobic, anaerobic, and microaerobic (flow-cell) conditions and in combination with the polymyxin antibiotic colistimethate sodium (colistin). HBED was assessed against strains of from patients with cystic fibrosis and the reference strain PAO1. HBED inhibited growth and biofilm formation of all clinical strains under aerobic and anaerobic conditions, but inhibitory effects against PAO1 were predominantly exerted under anaerobic conditions. PA605, which is a clinical strain with a robust biofilm-forming phenotype, was selected for flow-cell studies. HBED significantly reduced biomass and surface coverage of PA605, and, combined with colistin, HBED significantly enhanced the microcolony killing effects of colistin to result in almost complete removal of the biofilm. HBED combined with colistin is highly effective against biofilms formed by clinical strains of .
针对[具体细菌名称]的铁需求可能是传统抗生素治疗生物膜定植[具体细菌名称]的有效辅助手段。因此，我们评估了N,N'-双（2-羟基苄基）乙二胺-N,N'-二乙酸（HBED）的抗生物膜活性，它是一种合成的六齿铁螯合剂。在需氧、厌氧和微需氧（流动细胞）条件下，并与多粘菌素抗生素粘菌素甲磺酸钠（多粘菌素）联合使用，利用短期（微量滴定板）和长期（流动细胞）生物膜模型研究了HBED的作用。针对囊性纤维化患者的[具体细菌名称]菌株和参考菌株PAO1评估了HBED。在需氧和厌氧条件下，HBED抑制了所有临床菌株的生长和生物膜形成，但对PAO1的抑制作用主要在厌氧条件下发挥。选择具有强大生物膜形成表型的临床菌株PA605进行流动细胞研究。HBED显著降低了PA605的生物量和表面覆盖率，并且与多粘菌素联合使用时，HBED显著增强了多粘菌素对微菌落的杀伤作用，几乎导致生物膜完全清除。HBED与多粘菌素联合使用对[具体细菌名称]临床菌株形成的生物膜非常有效。
