Si Wenyu, Li Hailing, Kang Tiezhu, Ye Jing, Yao Zhiqiu, Liu Ya, Yu Tong, Zhang Yunhai, Ling Yinghui, Cao Hongguo, Wang Juhua, Li Yunsheng, Fang Fugui
Anhui Provincial Laboratory of Animal Genetic Resources Protection and Breeding, College of Animal Science and Technology, Anhui Agricultural University, 130 Changjiang West Road, Hefei 230036, China.
Anhui Provincial Laboratory for Local Livestock and Poultry Genetic Resource Conservation and Bio-Breeding, 130 Changjiang West Road, Hefei 230036, China.
Animals (Basel). 2020 Mar 27;10(4):567. doi: 10.3390/ani10040567.
This study explored the role of γ-aminobutyric acid transaminase (GABA-T) in the puberty and reproductive performance of female rats. Immunofluorescence technique, quantitative real-time PCR (RT-qPCR) and enzyme-linked immunosorbent assay (ELISA) were used to detect the distribution of GABA-T and the expression of genes and hormones in female rats, respectively. The results showed that GABA-T was mainly distributed in the arcuate nucleus (ARC), paraventricular nucleus (PVN) and periventricular nucleus (PeN) of the hypothalamus, and in the adenohypophysis, ovarian granulosa cells and oocytes. mRNA level at 28 d was lowest in the hypothalamus and the pituitary; at puberty, it was lowest in the ovary. mRNA level was highest in adults in the hypothalamus; at infancy and puberty, it was highest in the pituitary; and at 21 d it was highest in the ovary. After vigabatrin (GABA-T irreversible inhibitor) was added to hypothalamus cells, the levels of mRNA and mRNA were significantly reduced, but mRNA increased at the dose of 25 and 50 μg/mL; mRNA was significantly increased but mRNA was reduced at the 50 μg/mL dose. In prepubertal rats injected with vigabatrin, puberty onset was delayed. mRNA, mRNA and mRNA levels were significantly reduced, but mRNA level increased in the hypothalamus. Vigabatrin reduced the concentrations of GABA-T, luteinizing hormone (LH) and progesterone (P), and the ovarian index. Lactation performance was reduced in adult rats with vigabatrin treatment. Four hours after vigabatrin injection, the concentrations of GABA-T and LH were significantly reduced in adult and 25 d rats, but follicle-stimulating hormone (FSH) increased in 25 d rats. In conclusion, GABA-T affects the reproductive function of female rats by regulating the levels of , and in the hypothalamus as well as the concentrations of LH and P.
本研究探讨了γ-氨基丁酸转氨酶（GABA-T）在雌性大鼠青春期及生殖性能中的作用。分别采用免疫荧光技术、定量实时聚合酶链反应（RT-qPCR）和酶联免疫吸附测定（ELISA）检测雌性大鼠中GABA-T的分布以及基因和激素的表达。结果显示，GABA-T主要分布于下丘脑的弓状核（ARC）、室旁核（PVN）和室周核（PeN），以及腺垂体、卵巢颗粒细胞和卵母细胞中。28日龄时，下丘脑和垂体中的mRNA水平最低；在青春期，卵巢中的mRNA水平最低。成年时下丘脑的mRNA水平最高；在婴儿期和青春期，垂体中的mRNA水平最高；在21日龄时，卵巢中的mRNA水平最高。向下丘脑细胞中加入氨己烯酸（GABA-T不可逆抑制剂）后，mRNA和mRNA的水平显著降低，但在25和50μg/mL剂量时mRNA增加；在50μg/mL剂量时mRNA显著增加但mRNA降低。给青春期前大鼠注射氨己烯酸后，青春期启动延迟。下丘脑的mRNA、mRNA和mRNA水平显著降低，但mRNA水平升高。氨己烯酸降低了GABA-T、黄体生成素（LH）和孕酮（P）的浓度以及卵巢指数。用氨己烯酸处理的成年大鼠的泌乳性能降低。注射氨己烯酸4小时后，成年大鼠和25日龄大鼠的GABA-T和LH浓度显著降低，但25日龄大鼠的促卵泡激素（FSH）增加。总之，GABA-T通过调节下丘脑、和的水平以及LH和P的浓度来影响雌性大鼠的生殖功能。
