Servicio de Microbiología, Hospital Universitario Puerta del Mar, Facultad de Medicina, Universidad de Cádiz, Instituto para la Investigación e Innovación en Ciencias Biomédicas de Cádiz (INiBICA), Cádiz, Spain.
Servicio de Medicina Interna, Unidad de Enfermedades Infecciosas, Hospital Universitario Puerta del Mar, Facultad de Medicina, Universidad de Cádiz, Instituto para la Investigación e Innovación en Ciencias Biomédicas de Cádiz (INiBICA), Cádiz, Spain.
Eur J Clin Microbiol Infect Dis. 2020 Aug;39(8):1503-1512. doi: 10.1007/s10096-020-03875-y. Epub 2020 Mar 31.
Human immunodeficiency virus (HIV) antibodies have been proposed as a measure of the size of the HIV reservoir. The aim of our study is to quantify the anti-HIV antibodies level in a cohort of people living with HIV (PLWH), stratified based on the presence of continuous undetectable HIV viral load and the co-existence of hepatitis C virus infection. A sample of 229 HIV-monoinfected (n = 114) or HIV/HCV-coinfected [either with resolved HCV infection (n = 75) or active HCV coinfection (n = 40)] patients, followed up a median of 34 (IQR 20-44) months, was studied. Anti-HIV index was obtained as the 1:800 dilution of HIV antibodies. CD4+ T cell count, time with undetectable HIV viral load, annual increase of CD4+ T cell count, anti-HCV therapy, and diagnosis of cirrhosis were analyzed. Patients with a continued suppressed HIV viral load had significant lower anti-HIV index compared with those with virologic failure during the follow-up. Significant higher CD4+ T cell increase was observed in those with a lower anti-HIV index. HIV-monoinfected patients showed an anti-HIV index significantly lower than patients with HCV coinfection. Resolved HCV infection after interferon-based therapy, but not with direct acting antivirals, was associated with a lower anti-HIV index. HIV/HCV-coinfected patients showed higher HIV antibodies level when compared with HIV-monoinfected individuals. A decrease in anti-HIV index in HIV/HCV-coinfected patients was detected when a sustained virological HCV response was obtained after interferon-based therapy, in possible relation with the direct or indirect effect of interferon on PLWH CD4 T cells.
人类免疫缺陷病毒（HIV）抗体被提议作为 HIV 储存库大小的衡量标准。我们研究的目的是定量分析一组 HIV 感染者（PLWH）的抗 HIV 抗体水平，这些患者根据持续不可检测的 HIV 病毒载量和丙型肝炎病毒（HCV）感染的共存情况进行分层。研究了 229 例 HIV 单一感染（n = 114）或 HIV/HCV 合并感染[包括已解决的 HCV 感染（n = 75）或活跃的 HCV 合并感染（n = 40）]患者的样本，中位随访时间为 34 个月（IQR 20-44）。抗 HIV 指数是通过将 HIV 抗体稀释 1:800 获得的。分析了 CD4+T 细胞计数、持续不可检测的 HIV 病毒载量时间、CD4+T 细胞计数的年增长率、抗 HCV 治疗和肝硬化诊断。与随访期间病毒学失败的患者相比，持续抑制 HIV 病毒载量的患者的抗 HIV 指数显著降低。抗 HIV 指数较低的患者 CD4+T 细胞增加显著较高。与 HCV 合并感染的患者相比，HIV 单一感染患者的抗 HIV 指数显著降低。基于干扰素的治疗后获得 HCV 病毒学应答（但不是直接作用抗病毒药物）与较低的抗 HIV 指数相关。与 HIV 单一感染患者相比，HIV/HCV 合并感染患者的 HIV 抗体水平更高。在基于干扰素的治疗后获得持续病毒学 HCV 应答时，检测到 HIV/HCV 合并感染患者的抗 HIV 指数下降，这可能与干扰素对 PLWH CD4+T 细胞的直接或间接作用有关。
