Dong Yang, Wang Qi, Zheng Yan-Fei, Ma Jin-Chen, Li Bo-Yi, Wang Ji
School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China.
Zhonghua Nan Ke Xue. 2019 Nov;25(11):1015-1020.
To explore the regulatory effect of Huatan Qushi Decoction (HTQSD) on lipid metabolism and semen quality in obese rats with oligoasthenozoospermia (OA) and its underlying mechanisms.
Ninety-six 8-week-old male Wistar rats were randomly divided into six groups of equal number: blank control, OA model control, Wuzi Yanzong Pills (WZYZP), and high-, medium- and low-dose HTQSD, the blank controls fed with a normal diet and the others with a high-fat diet, all for 8 weeks. Meanwhile, the rats in the blank control and OA model control groups were treated intragastrically with normal saline, those in the WZYZP group with WZYZP at 1.07 g/kg, and those in the high-, medium- and low-dose HTQSD groups with HTQSD at 26.25, 13.125 and 6.5625 g/kg, respectively. The body weights of the animals were obtained at 1, 4 and 8 weeks of modeling and intervention, the levels of serum TG, TC, HDL-C and LDL-C, the liver and testis indexes and semen quality detected at 4 and 8 weeks, and the expression of HO-1 mRNA determined at 8 weeks.
At 8 weeks of modeling and intervention, the rats in the model control group, compared with the blank controls, showed significant increases in the body weight (［523.1 ± 25.54］ vs ［451.50 ± 27.53］ g, P < 0.01) and levels of serum TG (［8.58 ± 0.39］ vs ［2.18 ± 0.28］ nmol/L, P < 0.001), TC (［4.41 ± 1.44］ vs ［1.68 ± 0.18］ nmol/L, P < 0.01) and LDL-C (［2.06 ± 0.17］ vs ［0.48 ± 0.57］ nmol/L,P<0.01), but decreases in the level of serum HDL-C (［27.92 ± 0.40］ vs ［57.47 ± 1.52］ nmol/L,P < 0.01), sperm motility (［11.31 ± 4.87］% vs ［39.66 ± 2.77］%, P < 0.01), sperm concentration (［31.07 ± 10.52］ vs ［65.37 ± 6.30］ ×10⁶/ml, P < 0.01) and the expression of HO-1 mRNA (0.16 ± 0.03 vs 1.06 ± 0.20, P < 0.01). The rats in the medium-dose HTQSD group, in comparison with the model controls, exhibited remarkable decreases in the body weight (［445.13 ± 34.19］ vs ［523.1 ± 25.54］ g, P < 0.01) and levels of serum TG (［2.05 ± 0.27］ vs ［8.58 ± 0.39］ nmol/L,P < 0.01), TC (［1.63 ± 0.21］ vs ［4.41 ± 1.44］ nmol/L,P < 0.01) and LDL-C (0.45 ± 0.07) vs ［2.06 ± 0.17］ nmol/L, P < 0.01), but increases in the level of serum HDL-C (［48.35 ± 3.63］ vs ［27.92 ± 0.40］ nmol/L, P < 0.01), sperm motility (［32.84 ± 6.22］% vs ［11.31 ± 4.877］%, P < 0.01) and sperm concentration (［46.90 ± 6.39］ vs ［31.07 ± 10.52］ ×10⁶/ml, P < 0.05), and the low-dose HTQSD group showed a significantly up-regulated expression of HO-1 mRNA (0.76 ± 0.13 vs 0.16±0.03, P < 0.01).
HTQSD can regulate the blood lipid level and improve semen quality in obese rats with oligoasthenozoospermia by promoting serum anti-oxidation and reducing oxidative stress.
探讨化痰祛湿方对肥胖型少弱精子症（OA）大鼠脂质代谢和精液质量的调节作用及其潜在机制。
将96只8周龄雄性Wistar大鼠随机分为6组，每组数量相等：空白对照组、OA模型对照组、五子衍宗丸组（WZYZP）以及高、中、低剂量化痰祛湿方组。空白对照组给予正常饮食，其他组给予高脂饮食，持续8周。同时，空白对照组和OA模型对照组大鼠灌胃生理盐水，WZYZP组大鼠灌胃1.07 g/kg的WZYZP，高、中、低剂量化痰祛湿方组大鼠分别灌胃26.25、13.125和6.5625 g/kg的化痰祛湿方。在建模和干预的第1、4和8周测量动物体重，在第4和8周检测血清TG、TC、HDL-C和LDL-C水平、肝脏和睾丸指数以及精液质量，并在第8周测定HO-1 mRNA的表达。
建模和干预8周时，与空白对照组相比，模型对照组大鼠体重显著增加（［523.1±25.54］vs［451.50±27.53］g，P<0.01），血清TG（［8.58±0.39］vs［2.18±0.28］nmol/L，P<0.001）、TC（［4.41±1.44］vs［1.68±0.18］nmol/L，P<0.01）和LDL-C（［2.06±0.17］vs［0.48±0.57］nmol/L，P<0.01）水平升高，但血清HDL-C水平降低（［27.92±0.40］vs［57.47±1.52］nmol/L，P<0.01），精子活力（［11.31±4.87］% vs［39.66±2.77］%，P<0.01）、精子浓度（［31.07±10.52］vs［65.37±6.30］×10⁶/ml，P<0.01）以及HO-1 mRNA表达（0.16±0.03 vs 1.06±0.20，P<0.01）下降。与模型对照组相比，中剂量化痰祛湿方组大鼠体重（［445.13±34.19］vs［523.1±25.54］g，P<0.01）、血清TG（［2.05±0.27］vs［8.58±0.39］nmol/L，P<0.01）、TC（［1.63±0.21］vs［4.41±1.44］nmol/L，P<
