慢性酒精中毒动物模型中骨髓间充质干细胞干预坐骨神经的力学特性
Mechanical characteristics of BMSCs-intervened sciatic nerve in chronic alcohol-intoxicated animal model.
作者信息
Li Peng, Chen Yudong, Yang Kun, Chen Dachuan, Kong Daliang
机构信息
Department of Mechanics, School of Mechanical and Aerospace Engineering, Jilin University, Changchun, China.
Aviation Fundamental College, Aviation University of Air Force, Changchun, China.
出版信息
Int J Neurosci. 2021 Jul;131(7):650-656. doi: 10.1080/00207454.2020.1750397. Epub 2020 Apr 15.
OBJECTIVE
To study the mechanical properties of sciatic nerve in rats with chronic alcoholism (CA) and intervened with bone marrow mesenchymal stem cells (BMSCs) and to provide biomechanical basis for clinical practice.
METHODS
the serum of the BMSCs-intervened CA rats was sampled and determined the contents of malondialdehyde (MDA), metallothionein (CAS, MT), and Glutathione/r -glutamyl cysteinyl/glycine (GSH); meanwhile, the rats' sciatic nerve was tested the tensile and observed the histomorphological changes.
RESULTS
The mechanical properties of sciatic nerve in BMSCs-intervened CA rats, as well as the serum levels of MT and GSH, were significantly different from those in the basic fibroblast growth factor (bFGF)-intervened CA rats ( < 0.05).
CONCLUSIONS
BMSCs intervention can restore the levels of MT, GSH, MDA, histomorphology, and tensile mechanical properties in CA animal model, and its effects on repairing sciatic nerve are obvious.
目的
研究慢性酒精中毒（CA）大鼠经骨髓间充质干细胞（BMSCs）干预后坐骨神经的力学性能，为临床实践提供生物力学依据。
方法
采集经BMSCs干预的CA大鼠血清，测定丙二醛（MDA）、金属硫蛋白（CAS、MT）和谷胱甘肽/γ-谷氨酰半胱氨酸/甘氨酸（GSH）含量；同时对大鼠坐骨神经进行拉伸测试并观察组织形态学变化。
结果
经BMSCs干预的CA大鼠坐骨神经力学性能以及MT和GSH血清水平与经碱性成纤维细胞生长因子（bFGF）干预的CA大鼠相比有显著差异（<0.05）。
结论
BMSCs干预可恢复CA动物模型中MT、GSH、MDA水平、组织形态学及拉伸力学性能，其对坐骨神经的修复作用明显。
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