Namiki M, Nakamura M, Okuyama A, Sonoda T
Department of Urology, Osaka University Medical School.
Hinyokika Kiyo. 1988 Jun;34(6):945-9.
We established the assay conditions for human testicular FSH receptors using testicular tissues obtained from ten patients with prostate cancer. Based on the results of the time course study on specific FSH binding with increasing concentrations of the testicular homogenate at various temperatures, we found that the incubation was suitable at 25 degrees C for 20 hours using 10 mg wet tissue per tube. Scatchard analysis of specific FSH binding sites showed that FSH receptors in the human testicular tissue consisted of two classes of binding sites, one with a high affinity (Ka, 10(9) M-1) and the other with a low affinity (Ka, 10(8) M-1).
我们利用从10名前列腺癌患者获取的睾丸组织，建立了人睾丸促卵泡激素（FSH）受体的检测条件。基于在不同温度下，随着睾丸匀浆浓度增加对特异性FSH结合进行的时间进程研究结果，我们发现，每管使用10mg湿组织，在25℃孵育20小时是合适的。对特异性FSH结合位点的Scatchard分析表明，人睾丸组织中的FSH受体由两类结合位点组成，一类具有高亲和力（Ka，10⁹ M⁻¹），另一类具有低亲和力（Ka，10⁸ M⁻¹）。
