Department of Biochemical Diagnostics, Medical University, Bialystok, Poland
2 Department of Obstetrics and Gynecology, Medical University, Warsaw, Poland.
Anticancer Res. 2020 Apr;40(4):1997-2001. doi: 10.21873/anticanres.14155.
BACKGROUND/AIM: The liver of pregnant women undergoes physiological and pathological changes and the changes in liver enzyme activity and release reflect changes in serum enzymatic activity. We aimed to assess the activity of alcohol dehydrogenase (ADH) isoenzymes, and aldehyde dehydrogenase (ALDH) in the sera of women with intrahepatic cholestasis of pregnancy (ICP), the most common pregnancy-related liver disease.
Serum samples were taken from 40 women with ICP in the second or third trimester of pregnancy. Serum samples were also obtained from 40 healthy pregnant women at the same time of pregnancy and 40 healthy non-pregnant women. Class I and II of ADH and ALDH activity was measured by a spectrofluorometric method. Class III, IV ADH and total ADH activity was measured by photometric methods.
The total ADH activity was significantly higher in women with ICP than in healthy pregnant and non-pregnant women (about 42%). The median total activity of ADH was 1067 mU/l in women with ICP, 628 mU/l in healthy pregnant and 605 mU/l in non-pregnant women. A statistically significant increase in class I ADH isoenzymes was found in the sera of pregnant women with ICP. The median activity of this class in the ICP group increased about 62% and 80% in comparison to the healthy pregnant women and non-pregnant women, respectively.
The activity of class I ADH isoenzymes in the sera of women with ICP is statistically significantly increased and may have a diagnostic significance.
背景/目的：孕妇的肝脏会发生生理和病理变化，而肝酶活性和释放的变化反映了血清酶活性的变化。我们旨在评估妊娠期肝内胆汁淤积症（ICP）患者血清中醇脱氢酶（ADH）同工酶和醛脱氢酶（ALDH）的活性，ICP 是最常见的妊娠相关肝病。
采集 40 名妊娠中期或晚期 ICP 孕妇的血清样本。同时采集 40 名健康孕妇和 40 名健康未孕妇女的血清样本。采用荧光分光光度法测定 ADH 同工酶和 ALDH 活性的 I 型和 II 型。采用分光光度法测定 ADH 同工酶 III 型、IV 型和总 ADH 活性。
ICP 患者的总 ADH 活性明显高于健康孕妇和未孕妇女（约 42%）。ICP 患者的总 ADH 活性中位数为 1067 mU/l，健康孕妇为 628 mU/l，未孕妇女为 605 mU/l。ICP 孕妇血清中 I 型 ADH 同工酶活性明显升高。该类别的中位数活性与健康孕妇相比增加了约 62%，与未孕妇女相比增加了约 80%。
ICP 患者血清中 I 型 ADH 同工酶的活性显著增加，可能具有诊断意义。
