2nd Department of Obstetrics and Gynecology, Medical University of Warsaw, Warsaw, Poland.
Department of Biochemical Diagnostics, Medical University, Waszyngtona 15 A, 15-269, Bialystok, Poland.
Sci Rep. 2022 Jun 30;12(1):11071. doi: 10.1038/s41598-022-15532-9.
Intrahepatic cholestasis of pregnancy (ICP) is an important pregnancy liver disorder. The alterations of different enzymes activity in the hepatocytes in the course of liver diseases are reflected in an increase in the activity of the corresponding enzymes in the blood. In present study we assayed the activity of alcohol dehydrogenase (ADH) and its isoenzyme in the serum of patients with ICP. Serum were collected from 100 pregnancies with ICP in the second or third trimester of pregnancy. Serum samples were also taken from 100 healthy pregnant women. The activity of ADH I was measured by spectrofluorometric method, ADH total was measured by photometric method. There was significant increase in the activity of ADH I (2.79 mU/l vs. 1.72 mU/l) and total ADH activity (1103 mU/l vs. 682 mU/l) in the sera of women with ICP compared to the healthy pregnant women. Importantly, the sensitivity and specificity of ADH I for diagnosis of ICP were 85% and 91%, respectively. Area under the Receiver Operating Curve for ADH I in ICP was 0.81. The activity of ADH I in the sera of women with ICP is statistically significantly increased, which may have a diagnostic significance for ICP patients.
妊娠期肝内胆汁淤积症（ICP）是一种重要的妊娠肝内疾病。在肝脏疾病过程中，肝细胞中不同酶活性的改变反映在血液中相应酶活性的增加。本研究检测了 ICP 患者血清中醇脱氢酶（ADH）及其同工酶的活性。采集了 100 例妊娠中期或晚期 ICP 妊娠患者的血清，同时采集了 100 例健康孕妇的血清。采用荧光分光光度法测定 ADH I 活性，采用分光光度法测定总 ADH 活性。与健康孕妇相比，ICP 患者血清中 ADH I（2.79 mU/l 比 1.72 mU/l）和总 ADH 活性（1103 mU/l 比 682 mU/l）显著增加。重要的是，ADH I 诊断 ICP 的灵敏度和特异性分别为 85%和 91%。ADH I 在 ICP 中的受试者工作特征曲线下面积为 0.81。ICP 患者血清中 ADH I 的活性显著增加，这可能对 ICP 患者具有诊断意义。
