Nahm Francis Sahngun, Lee Jae-Sung, Lee Pyung-Bok, Choi Eunjoo, Han Woong Ki, Nahm Sang-Soep
Department of Anesthesiology and Pain Medicine, Seoul National University Bundang Hospital, Seongnam, Korea.
Department of Anesthesiology and Pain Medicine, College of Medicine, Seoul National University, Seoul, Korea.
Korean J Pain. 2020 Apr 1;33(2):131-137. doi: 10.3344/kjp.2020.33.2.131.
Among various diseases that accompany pain, complex regional pain syndrome (CRPS) is one of the most frustrating for patients and physicians. Recently, many studies have shown functional and anatomical abnormalities in the brains of patients with CRPS. The calcium-related signaling pathway is important in various physiologic processes calmodulin (CaM) and calcium-calmodulin kinase 2 (CaMK2). To investigate the cerebral mechanism of CRPS, we measured changes in CaM and CaMK2 expression in the cerebrum in CRPS animal models.
The chronic post-ischemia pain model was employed for CRPS model generation. After generation of the animal models, the animals were categorized into three groups based on changes in the withdrawal threshold for the affected limb: CRPS-positive (P), CRPS-negative (N), and control (C) groups. Western blot analysis was performed to measure CaM and CaMK2 expression in the rat cerebrum.
Animals with a decreased withdrawal threshold (group P) showed a significant increment in cerebral CaM and CaMK2 expression ( = 0.013 and = 0.021, respectively). However, groups N and C showed no difference in CaM and CaMK2 expression.
The calcium-mediated cerebral process occurs after peripheral injury in CRPS, and there can be a relationship between the cerebrum and the pathogenesis of CRPS.
在伴有疼痛的各种疾病中，复杂性区域疼痛综合征（CRPS）是让患者和医生最为困扰的疾病之一。最近，许多研究表明CRPS患者大脑存在功能和解剖学异常。钙相关信号通路在各种生理过程中很重要，涉及钙调蛋白（CaM）和钙调蛋白激酶2（CaMK2）。为了研究CRPS的大脑机制，我们在CRPS动物模型中测量了大脑中CaM和CaMK2表达的变化。
采用慢性缺血后疼痛模型来建立CRPS模型。在建立动物模型后，根据患侧肢体退缩阈值的变化将动物分为三组：CRPS阳性（P）组、CRPS阴性（N）组和对照组（C）。进行蛋白质免疫印迹分析以测量大鼠大脑中CaM和CaMK2的表达。
退缩阈值降低的动物（P组）大脑中CaM和CaMK2表达显著增加（分别为 = 0.013和 = 0.021）。然而，N组和C组在CaM和CaMK2表达上没有差异。
在CRPS中，外周损伤后会发生钙介导的大脑过程，大脑与CRPS的发病机制之间可能存在关联。
