Fiore Enrico, Tessari Rossella, Morgante Massimo, Gianesella Matteo, Badon Tamara, Bedin Silvia, Mazzotta Elisa, Berlanda Michele
Department of Animal Medicine, Production and Health, University of Padova, Viale dell' Università 16, 35020 Legnaro (PD), Italy.
Animals (Basel). 2020 Mar 29;10(4):571. doi: 10.3390/ani10040571.
Excessive mobilization of adipose tissue in high milk producing dairy cows predisposes to metabolic diseases. The aim of this research was to identify the plasma fatty acids in four lipid classes as biomarkers for the diagnosis of hyperketonemia in bovines using thin layer chromatography and gas chromatographic techniques (TLC-GC). Sixty multiparous Holstein-Friesian dairy cows were enrolled in the study. Blood samples from the coccygeal vein were collected and β-hydroxybutyrate (BHB) was evaluated. All animals were divided into three groups on the basis of ketonemia: BHB < 0.50 mmol/L, 0.50 < BHB < 1.0 mmol/L, and BHB > 1.0 mmol/L. Plasma fatty acid concentrations were evaluated in four lipid classes: Free Fatty Acids (FFA), Triglycerides (TG), Cholesterol Esters (CE) And Phospholipids (PL). The concentration of fatty acids was analyzed using TLC-GC. The results showed the following significance in the lipid classes: 19 fatty acids were significant ( < 0.053) in FFA, nine fatty acids were significant ( < 0.050) in TG, eight fatty acids were significant ( < 0.050) in CE and three fatty acids were significant ( < 0.049) in PL. Eleven parameters were considered as predictive fatty acids related to animals in hyperketonemia. The FFA increased simultaneously with blood BHB levels, although the identified predictive fatty acids related to the TG and CE lipid classes decreased, meanwhile the BHB values increased. In the PL lipid class, no fatty acids were predictive.
高产奶牛脂肪组织过度动员易引发代谢疾病。本研究的目的是利用薄层色谱和气相色谱技术（TLC-GC），鉴定四类脂质中的血浆脂肪酸，作为牛酮血症诊断的生物标志物。60头经产荷斯坦-弗里生奶牛参与了本研究。采集尾静脉血样并评估β-羟基丁酸（BHB）。根据酮血症情况将所有动物分为三组：BHB < 0.50 mmol/L、0.50 < BHB < 1.0 mmol/L和BHB > 1.0 mmol/L。评估了四类脂质中的血浆脂肪酸浓度：游离脂肪酸（FFA）、甘油三酯（TG）、胆固醇酯（CE）和磷脂（PL）。使用TLC-GC分析脂肪酸浓度。结果显示脂质类别具有以下显著性：FFA中有19种脂肪酸显著（< 0.053），TG中有9种脂肪酸显著（< 0.050），CE中有8种脂肪酸显著（< 0.050），PL中有3种脂肪酸显著（< 0.049）。11个参数被视为与酮血症动物相关的预测性脂肪酸。FFA与血液BHB水平同时升高，尽管与TG和CE脂质类别相关的已鉴定预测性脂肪酸减少，而BHB值升高。在PL脂质类别中，没有脂肪酸具有预测性。
